摘要
【目的】氮污染已成为当今水体污染的一个重要因素,为了解滇池可培养好氧反硝化细菌的多样性,获得高效好氧反硝化细菌资源,为污染水体或浅层地下水的生物修复提供材料。【方法】采用富集培养方法从滇池沉积物和水体样品中分离好氧反硝化细菌,对好氧反硝化细菌的16S r RNA基因序列进行系统发育分析,并筛选其中的高效好氧反硝化细菌。【结果】分离出260株好氧反硝化菌,经16S rRNA基因序列分析,260株菌分属于2门13科14属的59个种。假单胞菌属(Pseudomonas)为优势细菌属,其次是不动杆菌属(Acinetobacter)、气单胞菌属(Aeromonas)和代尔夫特菌属(Delftia)。筛选到12株高效好氧反硝化细菌菌株,其中8株属于假单胞菌(Pseudomonas spp.),4株为不动杆菌(Acinetobacter spp.)。定量分析发现菌株N15-6-1的反硝化效果较好。对菌株N15-6-1的脱氮条件优化结果显示,在以蔗糖为碳源,温度为30–35℃、C/N=12、静止培养时,反硝化能力较强,其在48 h内硝态氮的去除率达到98.81%,总氮的去除率达96.27%。【结论】滇池存在着较丰富的可培养好氧反硝化细菌,好氧反硝化细菌的分离丰富了好氧反硝化菌的种类,其中的高效脱氮菌株为污染水体或浅层地下水的生物修复提供了初步的候选菌株。
[Objective] At present, nitrogen pollution has been becoming an important factor in water pollution. In order to unveil the diversity of culturable aerobic denitrifying bacteria in Dianchi Lake and obtain efficient aerobic denitrifying bacteria for providing evidence to bioremediate the polluted water or shallow groundwater. [Methods] Aerobic denitrifying bacteria were obtained through enrichment method and screening processes. The taxonomic positions of these aerobic denitrifying bacteria were analyzed based on 16 S r RNA gene sequence comparisons. The efficient aerobic denitrifying bacteria were screened out by detecting nitrogen removal efficiency. [Results] In this study, 260 strains of aerobic denitrifying bacteria were isolated from the sediment and water samples of Dianchi Lake. These bacteria were classified into 59 species, belonging to 14 genera of 13 families of in 2 phyla of bacteria. Pseudomonas composed the major culturable aerobic denitrifying bacteria of Dianchi Lake, followed by Acinetobacter,Aeromonas and Delftia. Twelve strains with perfect nitrogen removal characteristics were screened out. Therein, 8 strains were identified as Pseudomonas spp. and 4 strains were identified as Acinetobacter spp. One efficient denitrifying strain N15-6-1 was screened out by using the quantitative analysis. Its nitrate and total nitrogen removal rates reached 98.81% and 96.27% in 48 h at 30–35 ℃, C/N=12, repectively. [Conclusion] From all the results, the higher diversity of culturable aerobic denitrifying bacteria inhabited in Dianchi Lake, which enriched the species resources of aerobic denitrifying bacteria, and furthermore, the efficient aerobic denitrifying bacteria provided a significant candidate strains to bioremediate the polluted water or shallow groundwater.
作者
王永霞
霍晴晴
李亚平
肖炜
赖泳红
和树庒
崔晓龙
Yongxia Wang1, Qingqing Huo1, Yaping Li1, Wei Xiao1, Yonghong Lai1, Shuzhuang He2, Xiaolong Cui1,3.(1. Yunnan Institute of Microbiology, School of Life Sciences, Yunnan University, Kunming 650091, Yunnan Province, China; 2. School of Ecology and Environmental Science, Yunnan University, Kunming 650091, Yunnan Province, China; 3. State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan University, Kunming 650091, Yunnan Province, China)
出处
《微生物学报》
CAS
CSCD
北大核心
2018年第10期1764-1775,共12页
Acta Microbiologica Sinica
基金
国家重点科技计划项目水专项(2012ZX07102-003)
国家自然科学基金(31660001
31660089
31660042)
国家微生物资源平台专项-面向微生物教学实验的专题服务项目(NIMR-2016-8)~~
关键词
滇池
富集培养
好氧反硝化细菌
系统发育分析
脱氮特性
Dianchi lake
enrichment
aerobic denitrifying bacteria
phylogenetic analysis
nitrogen removal characteristic
