摘要
目的观察Glu、mGlu7拮抗剂MMPIP对正常大鼠海马CA3区痛觉的影响,探究Glu、MMPIP和海马CA3区在正常大鼠痛觉调制中的作用。方法 Wistar大鼠42只随机分为3组,分为生理盐水对照组14只、Glu组14只、MMPIP组14只,每个用药组痛反应神经元数量大致相等。采用电生理学的方法,以电脉冲刺激坐骨神经作为伤害性痛刺激。用玻璃微电极记录痛反应神经元放电的变化,并连续观察和记录30 min内痛反应神经元的电变化。结果 Glu使正常大鼠海马CA3区中PEN净增值(NIV)增加,潜伏期缩短,使PIN的NIV减少,抑制时程(ID)延长。MMPIP使正常大鼠海马CA3区中PEN净增值(NIV)减少,潜伏期延长,使PIN的NIV增加,抑制时程(ID)缩短。结论 Glu使正常大鼠海马CA3区中痛兴奋神经元和痛抑制神经元对伤害性刺激的反应均增强,均呈易化疼痛作用,而MMPIP作用相反,表现出镇痛效应。
Objective To study the effects of Glu and a selective mGluR7 allosteric antagonist MMPIP on the electrical activities in the hippocampal CA3 of normal rats,and to further investigate the algesic modulation of Glu,MMPIP and the hippocampal CA3 in normal rats. Methods Forty-two Wistar rats were randomly divided into physiological saline group（n = 14）,Glu group（n = 14） and MMPIP group（n = 14）,which the number of pain-related neurons in the three groups were almost the same. The electrophysiological method was used. The trains of electrical impulses applied to the sciatic nerve were used as noxious stimulation. The electrical changes of pain-related neuron in the hippocampal CA3 were recorded by the glass microelectrode. The electrical changes of pain-related neuron were continually observed and recorded for30 minutes. Results Pain-excited neurons（PENs） and pain-inhibited neurons（PINs） exist in the hippocampal CA3 of normal rats; Glu potentiated the electric activities of evoked discharges of PENs and simultaneously inhibited those of PINs in the hippocampal CA3 of normal rats,MMPIP inhibited the electric activities of evoked discharges of PEN and enhanced those of PIN in the hippocampal CA3 of normal rats. Conclusion Glu facilitates nociceptive effect,while the effect is reversed by the intra-hippocampal CA3 region injection of MMPIP.
出处
《哈尔滨医科大学学报》
CAS
2018年第4期309-317,共9页
Journal of Harbin Medical University
