摘要
目的：采用多指标综合评分法确定临床经验方稳心汤的最佳提取工艺。方法：采用L_9（3~4）正交试验设计法对稳心汤进行提取工艺优化,采用多指标综合评分法确定最优提取工艺。对姜黄、肉桂、莪术、细辛考察加水量、浸泡时间、提取时间对挥发油得率的影响;对党参、川芎、全蝎进行乙醇正交试验设计,以党参炔苷和干膏率进行综合评分;对赤芍、大黄、山楂、麦冬、甘草,以及经乙醇提取党参、川芎、全蝎残留的药渣进行水提醇沉工艺正交试验优化,以芍药苷和干膏率的综合评分为考察指标,优选出最佳工艺参数,并进行验证。结果：确定了稳心汤的提取工艺,其中姜黄、肉桂、莪术、细辛提取挥发油的最佳提取工艺为加入8倍量水,浸泡6 h,蒸馏5 h,挥发油的得率较高;对党参、川芎、全蝎加入乙醇提取,最佳工艺为加入10倍70%乙醇,提取1.5 h,提取2次;对乙醇提取药材残渣及赤芍、大黄、山楂、麦冬、甘草等进行水提醇沉,水提取工艺为加入8倍水,提取1.0 h,提取2次,醇沉工艺为药液质量浓度1.12 g·mL^（-1）,加入95%乙醇使含醇量达到60%,静止醇沉36 h。结论：通过多指标综合评分法优化的稳心汤提取工艺稳定可靠,适合工业化生产,为临床应用和新药的研发提供参考。
Objective： To determine the optimum extraction process of a clinical experience prescription,Wenxin decoction（ WXT）,by using multi-index integrated evaluation method. Method： Orthogonal test L_9（3~4）was conducted for the optimization of extraction technology and multi-index grading method was used to determine the optimal one. Curcumae Longae Rhizoma, Cinnamomi Cortex, Curcumae Rhizoma, and Asari Radix et Rhizoma were evaluated from the influence of amount of water,soaking time and extraction time on the yield rate.Orthogonal experiment design was applied in Codonopsis Radix,Chuanxiong Rhizoma and Scorpio with ethanol as solvent while lobetyolin and dry extract yield were used for overall scoring. Orthogonal experiment was used to optimize the water extraction and alcohol precipitation technologies for Paeoniae Radix Rubra, Rhei Radix et Rhizoma,Crataegi Fructus,Ophiopogonis Radix,Glycyrrhizae Radix et Rhizoma and residual dregs of Codonopsis Radix,Chuanxiong Rhizoma and Scorpio extracted by ethanol. Paeoniflorin and dry extract yield were chosen as the index of evaluation to select and verify the optimal process parameters. Result： The extraction process of WXT was determined. The optimal extraction process for higher yield of volatile oil from Curcumae Longae Rhizoma,Cinnamomi Cortex,Curcumae Rhizoma,and Asari Radix et Rhizoma was to soke these drugs for 6 hours with 8 times of water volume,and then distill those drugs for 5 hours. The optimal ethanol extraction process for Codonopsis Radix,Chuanxiong Rhizoma and Scorpio were as follows： 10 times of 80% ethanol amount,extracting for 2 times and 1. 5 hours each time. Water extraction and alcohol precipitation were used in Paeoniae Radix Rubra,Rhei Radix et Rhizoma,Crataegi Fructus,Ophiopogonis Radix,licorice and residual dregs. The water extraction process was 1 hour extraction for twice,with 8 times of water volume. The ethanol-precipitation process was established where the 95% ethanol was added up to 60% and precipitated for 36 hours in 1. 12 g·mL^（-1） liquid density. Conclusion： The extraction process of WXT optimized by multi-index integrated evaluation method is stable and reliable,which is suitable for industrial production,and can provide reference for clinical application and development of new drugs.
作者
陈恒文
田盼盼
何轩辉
马涛
李军
CHEN Heng-wen;TIAN Pan-pan;HE Xuan-hui;MA Tao;LI Jun(Guang＇anmen Hospital,China Academy of Chinese Medical Science,Beijing 100053,China;Beijing University of Chinese Medicine,Beijng 100029,China)
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2018年第20期25-31,共7页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家自然科学基金项目(81503421)
中央级公益性科研院所基本科研业务费专项(ZZ0808005)
国家中医药管理局国家中医临床研究基地业务建设科研专项(JDZX2015249)
中国科协青年人才托举工程(2017QNRC001)
关键词
稳心汤
多指标综合评分
正交试验
中药复方
Wenxin decoction
multi-index integrated evaluation method
orthogonal experimental
traditional Chinese medicine presciption
