摘要
苦瓜枯萎病是尖孢镰刀菌苦瓜专化型引起的真菌病害,探明苦瓜枯萎病的抗性遗传机制对制定抗病育种策略具有现实指导意义。本文以抗枯萎病苦瓜材料Thai4-6和感病材料CN19-1为亲本配制杂交组合,基于该组合6世代遗传群体(P_1、P_2、F_1、F_2、BCP_1和BCP_2),采用主基因+多基因混合遗传模型分析枯萎病抗性遗传特性。数据分析结果表明,该杂交组合的枯萎病抗性呈连续分布,最适模型为2对加性-显性-上位性主基因+加性-显性多基因遗传模型(E-1),2对主基因加性效应值均为–13.85,显性效应值分别是25.58和34.26,主基因遗传率在BCP_1、BCP_2和F_2中分别是86.03%、80.34%和94.25%,表明该组合枯萎病抗性主要受2对主基因控制。环境因素引起的变异在3个分离世代群体中分别占13.97%、14.06%和5.75%。本研究可为抗枯萎病苦瓜育种提供理论依据。
Bitter gourd wilt is a fungus disease caused by Fusarium oxyspoorum Schl. f. sp. momodicae Sun Huang. Exploring resistance mechanism has practical guiding significance for wilt resistance breeding of bitter gourd. Crosses had been made between bitter gourd line Thai4-6, high resistant to fusarium wilt and CN19-1, high susceptible to fusarium wilt. The inheritance of fusarium wilt resistance was analyzed with P1, P2, F1, BCP1, BCP2 and F2 populations through employing the major gene plus polygene mixed inheritance model. The results showed that the wilt resistance in the populations of F2, BCP1, and BCP2 was a continuous distribution. The most suitable model was E-1 model, two major genes with additive-dominance-epistasis effects plus polygenes with additive-dominance effects. The additive effect of the 2 major genes was –13.85 and the dominance effect was 25.58 and 34.26, respectively. The heritability value of the major genes in BCP1, BCP2, and F2 was 86.03%, 80.34% and 94.25%, respectively. The results showed that the wilt resistance was mainly controlled by 2 major genes. The environmental variation in BCP1, BCP2, and F2 accounted for 13.97%, 14.06% and 5.75%, respectively. This study could provide a theoretical basis for the wilt resistance breeding of bitter gourd.
作者
刘子记
朱婕
牛玉
杨衍
LIU Zijil;ZHU Jie;NIU Yu;YANG Yan(Tropical Crops Genetic Resources Institute,Chinese Academy of Tropical Agricultural Sciences,Danzhou,Hainan 571737,China;Institute of Tropical Agriculture and forestry,Hainan University,Haikou,Hainan 570228,China)
出处
《热带作物学报》
CSCD
北大核心
2018年第8期1501-1506,共6页
Chinese Journal of Tropical Crops
基金
国家自然科学基金项目(No.31601758)
海南省重点研发计划科技合作方向项目(No.ZDYF2016225)
"一带一路"项目(No.ZYLH2018010104)
关键词
苦瓜
混合遗传模型
枯萎病抗性
遗传分析
bitter gourd
wilt resistance
mixed genetic model
genetic analysis
