摘要
为明确羧基化壳寡糖络合碘(CCOS-I)对水稻纹枯病的防治作用,以20%井冈霉素可湿性粉剂为对照药剂,测定CCOS-I对水稻纹枯病菌的室内抑菌效果和田间防效,以及对超氧化物歧化酶(superoxidedismutase,SOD)、叶片过氧化物酶(peroridase,POD)、多酚氧化酶(polyphenol oxidase,PPO)、苯丙氨酸解氨酶(phenylalanine ammonia-lyase,PAL)和β-1,3-葡聚糖酶(β-1,3-glucanase)等相关防御酶活性的影响。结果表明:羧基化壳寡糖络合碘对该病菌抑制效果明显,经室内毒力测定,其EC50值12.22 mg/L,明显高于对照药剂20%井冈霉素粉剂。田间药效试验结果表明,在水稻第3次用药后14d,100g/hm2的羧基化壳寡糖络合碘防效达到80.66%,与井冈霉素有效剂量150 g/hm2的效果相当,优于同剂量井冈霉素处理。CCOS-I在试验剂量范围内对水稻纹枯病有很好的防治作用,且对水稻生长无任何药害现在发生,可以诱导水稻相关防御酶活性提高,适用于水稻纹枯病的防治,具有一定的开发推广价值。
In order to clarify the preventive and therapeutic effects on rice sheath blight, 20% validamycin wettable powder as the control, the antibacterial effect of carboxylated chitosan oligosaccharide iodine complex（CCOS-I） on R.solani in a greenhouse and its efficacy in the paddy field were assayed. And the induced activity of CCOS-I on the peroxidase（POD）, superoxide dismutase（SOD）, polyphenol oxidase（PPO）, phenylalnaine ammonia-lyase（PAL） and beta-1,3-glucanase（beta-1,3-glucanase） were studied. CCOS-I had a significant inhibitory effect on R. solani, and its EC50 value was 12.22 mg/L, which was obviously higher than that of the control in the field trial test, The control effect of CCOS-I at the effective dose of 100 g/hm^2 was 80.66%,which was equivalent to the valid dose of validamycin 150 g/hm^2 and better than the same dose of validamycin,after the third spraying 14 d. Compared with the spraying control agent, CCOS-I not only had good control effect on rice sheath blight, but also had a certain promoting effect on rice growth. CCOS-I induced rice resistance against R. solani which related to the increased activities of defense enzymes caused by CCOS-I. CCOS-I was safe for rice and had a certain value of development and promotion.
作者
胡雪芳
田志清
梁亮
朱祥民
姜昊业
王士奎
裴海生
HU Xuefang;TIAN Zhiqing;LIANG Liang;ZHU Xiangmin;JIANG Haoye;WANG Shikui;PEI Haisheng(Institute of Agricultural Products Processing Engineering Research,Chinese Academy of Agricultural Engineering Planning ＆ Design,Beijing 100125,China;Key Laboratory of Agro-Products Postharvest Handling,Ministry of Agriculture and Rural Affairs,Beijing 100121,China;Shanghai Guanfa Marine Biological Technology Co.Ltd.,Shanghai 200052,China)
出处
《热带作物学报》
CSCD
北大核心
2018年第8期1590-1595,共6页
Chinese Journal of Tropical Crops
基金
"十二五"国家科技支撑计划项目"海洋生物资源综合利用技术"(No.2013BAB01B00)
北部湾海洋生物资源系列特色产品关键技术研发与应用示范项目
