摘要
为明确内蒙古西部地区放线菌的多样性,为未来该地区放线菌资源的开发利用及生态系统的稳定性提供一定的理论基础。本研究从内蒙古西部地区采集土壤样品,提取土壤样品的总DNA,通过巢式PCR扩增放线菌16S rRNA基因片段,利用DGGE （denaturing gradinent gel electrophoresis）技术进行分析,切胶回收,再利用不带GC夹子的引物进行扩增,将PCR产物送往公司测序后对16S rRNA基因序列进行比对,分析内蒙古西部地区放线菌的多样性及其与土壤理化性质之间的相关性。对内蒙古西部地区放线菌多样性分析发现,内蒙古西部地区土壤放线菌的多样性较为丰富,丰富度指数处于10～28之间,其中,棕钙土的丰富度最高,栗褐土的最低;同一地区相同类型土壤中草地的放线菌数量比耕地丰富。放线菌的多样性与有效磷的含量呈显著正相关。经过16S rRNA基因序列比对共得到了7个属的放线菌,包括Saccharopolyspora （糖多孢菌属）、Thermomonospora （高温单孢菌属）、Streptomyces （链霉菌属）、Iamiaceae （酸微菌属）、Corynebacterium （棒杆菌属）、Timonella、Pseudonocardia （假诺卡氏菌属）。通过研究得出内蒙古西部地区的放线菌资源较为丰富,作为应用于抗细菌、抗真菌和抗肿瘤的新型药物中的重要微生物资源,具有进一步研究的价值,继而推动内蒙古西部地区放线菌资源的开发与利用。
The purpose of this study was to clarify the diversity of soil actinomycetes, and in the western region of Inner Mongolia were clarified, which provide the certain theoretical basis for the future development and utilization of actinomycetes and the stability of ecosystem. Soil samples were collected from the western region of Inner Mongolia and the total DNA was extracted from the soil samples. Then, 16S rRNA gene fragments of actinomycetes were amplified by the nested PCR and analyzed by DGGE （Denaturing Gradinent Gel Electrophore- sis） technique. DNA fragments on the gel were amplified again with the primers without GC clip and sequenced to analyze the diversity of actinomycetes in the western region of Inner Mongolia and its correlation with the physical and chemical properties of soil samples. The diversity analysis showed that the diversity of actinomycetes in the western region of Inner Mongolia was relatively rich. The richness index was between 10 and 28. The abundanceof actinomycetes of Brown pedocals was the highest and that of Castano-cinnamon soils was the lowest. The actinomycetes of grass lands were more abundant than that of the cultivated lands for the same soil types in the same area. The diversity of actinomycetes was significantly positively associated with the content of the effective phosphorus, A total of 7 genera of actinomycetes were obtained by 16S rRNA gene sequence analysis, including Saccharopolyspor, Thermomonospora, Streptomyces, Iamiaceae, Corynebacterium, TimoneUa, Pseudonocardia. It was concluded that actinomycetes resources in the western region of Inner Mongolia were abundant, which have the further research value as the important microorganism resources for the development of the new antibacterial, antifungal, and antitumor drugs.
作者
王雪寒
夏冬双
武志华
任兴波
丁一秀
马强
刘惠荣
Wang Xuehan;Xia Dongshuang;Wu Zhihua;Ren Xingbo;Ding Yixiu;Ma Qiang;Liu Huirong(College of Life Sciences,Inner Mongolia Agricultural University,Hohhot,010018)
出处
《基因组学与应用生物学》
CAS
CSCD
北大核心
2018年第10期4340-4348,共9页
Genomics and Applied Biology
基金
国家自然科学基金(No.31370058)资助
