摘要
通过普通野生稻转录组文库筛选到一个普通野生稻(Oryza rufipogon Griff.)根特异表达基因,并克隆启动子序列,命名为Or RSGp,长度为896 bp,含有CAAT-box、TATA-box等主要功能元件,还有参与茉莉酸甲酯反应的响应元件,以及抗旱、抗逆等响应元件。将其与gus报告基因融合转化拟南芥,GUS组织化学染色与定量分析结果表明,Or RSGp调控gus基因在根部特异表达。
Based on previous transcriptome data, a root-specific expression gene of common wild rice （ Oryza rufipogon Griff. ） was screened, and the promoter sequence was cloned and designated as OrRSGp. It was 896 bp in length and contained main functional elements such as CAAT-box and TATA-box, MeJA-responsiveness and some elements involved in defense and stress responsiveness. It was fused with the β-glucuronidas （ gus ） reporter gene and transformed into Arabidopsis thaliana. GUS histochemical staining and quantitative analysis showed that OrRSGp regulated gene gus specific expression in roots.
作者
黄珂
黄格格
薛满德
龙艳
袁潜华
裴新梧
HUANG Ke;HUANG Ge-ge;XUE Man-de;LONG Yan;YUAN Qian-hua;PEI Xin-wu(College of Tropical Agriculture and Forestry,Hainan University,Haikou 570228;MOA Key Laboratory on Safety Assessment（Molecular）of Agri-GMO,Chinese Academy of Agricultural Sciences,Beifing 100081)
出处
《生物技术通报》
CAS
CSCD
北大核心
2018年第8期87-92,共6页
Biotechnology Bulletin
基金
转基因重大专项(2016ZX08011-001)
国家自然科学基金项目(31260153)
关键词
普通野生稻
启动子
根特异表达
common wild rice
promoter
root tissue-specific expression
