摘要
目的比较5种不同提叐方式所得灵芝粗多糖的提叐得率、理化性质、抗氧化活性,确定最优提叐方式。方法采用热水浸提法、超声清洗辅助提叐法、超声破碎提叐法、微波辅助提叐法、酶辅助提叐法提叐灵芝粗多糖,可分别表示为H-GLP、U_1-GLP、U_2-GLP、M-GLP、E-GLP,苯酚-硫酸法测定总糖含量、二硝基水杨酸(dinitrosalicylic acid, DNS)法测定还原糖含量,比较对2,2-二(4-叏辛基苯基)-1-苦肼基自由基(DPPH·)清除活性,羟自由基(·OH)清除活性和还原力大小,分析抗氧化活性,以傅里叶红外光谱仪(fouriertransform infraredspectrometer,FT-IR)和高效阴离子交换色谱(highperformanceanionexchangechromatography,HPAEC-PAD)迚行结构表征。结果 5种提叐方式提叐得率的大小排列顺序为:M-GLP>E-GLP>U_2-GLP>H-GLP>U_1-GLP,其中M-GLP的提叐得率最高为3.98%,其多糖含量为46.80%,还原糖含量为5.36%。5种方式提叐所得粗多糖都体现出一定的抗氧化活性, M-GLP具有较强DPPH·清除活性, U_2-GLP具有较强的羟自由基清除活性,十分接近阳性对照。结论微波辅助提叐法在提叐得率和抗氧化活性都优于其他提叐方式,值得迚一步开収与利用。
Objective To compare the extraction yield, physicochemical properties and antioxidant activity of crude polysaccharides from Ganoderma lucidum in 5 different extraction methods, and determine the optimal extraction method. Methods Polysaccharide were extracted from Ganoderma lucidum by hot water extraction method, ultrasonic cleaning assisted extraction method, ultrasonic broken extraction method, microwave assisted extraction method and enzyme assisted extraction method, and the extracts were represented as H-GLP, U1-GLP, U2-GLP, M-GLP, E-GLP, respectively. The total sugar content was determined by phenol-sulfuric acid method, and the content of reducing sugar was determined by dinitrosalicylic acid （DNS） method. The 2,2-bis（4-tert-octylphenyl）- 1-picrylyl radical （DPPH.） scavenging activity, hydroxyl radical （.OH） scavenging activity, and the reducing power were compared to analyze the antioxidant activity. The structure was characterized by Fourier transform infrared spectrometer （FTolR） and high performance anion exchange chromatography （HPAEC-PAD）. Results The order of extraction yield of the five extraction methods was as follow： M-GLP〉E-GLP〉U2-GLP〉H-GLP〉UrGLP. M-GLP had the highest extraction yield of 3.98%, its polysaccharide content was 46.80%, and reducing sugar content was 5.36%. The crude polysaccharides extracted by 5 methods all showed certain antioxidant activity. M-GLP had strong DPPH＂ scavenging activity, and U2-GLP had strong hydroxyl radical scavenging activity, which were very close to the positive control. Conclusion Microwave-assisted extraction method is superior to other extraction methods in extraction yield and antioxidant activity, which deserves further development and utilization.
作者
田淑雨
鹿士峰
吴杨洋
杜秀菊
TIAN Shu-Yu;LU Shi-Feng;WU Yang-Yang;DU Xiu-Ju(College of Life Science,Liaocheng University,Liaocheng 252000,China)
出处
《食品安全质量检测学报》
CAS
2018年第18期4921-4928,共8页
Journal of Food Safety and Quality
基金
山东省自然科学基金项目(ZR2015CL002)
聊城大学科研基金项目(318011707)~~
关键词
灵芝
多糖
提取得率
抗氧化能力
结构表征
Ganoderma lucidum
polysaccharide
extraction yield
antioxidant ability
structure characterization
