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HPLC法同时测定心宁片中没食子酸、丹参素、原儿茶醛及芍药苷的含量 被引量:4

HPLC Simultaneous Determination of Gallic Acid,Tanshinol,Protocatechuic aldehyde and Paeoniflorin in Xinning Tablets
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摘要 目的建立同时测定心宁片中没食子酸、丹参素、原儿茶醛及芍药苷含量的高效液相色谱(HPLC)法。方法采用Altima C18(250 mm×4.6 mm,5μm)色谱柱,流动相为甲醇-0.15%磷酸溶液,流速1.0 m L·min-1,柱温35℃,检测波长分别为273 nm、283 nm、280 nm及230 nm。结果没食子酸浓度在0.011 2~0.180 2 mg·mL^(-1)范围内(r=1.000 0),丹参素浓度在0.012 5~0.201 3 mg·mL^(-1)范围内(r=1.000 0),原儿茶醛浓度在0.001 2~0.0201 mg·mL^(-1)范围内(r=1.000 0),芍药苷浓度在0.0124~0.199 1mg·mL^(-1)范围内(r=1.000 0),与峰面积均呈良好的线性关系;平均回收率(n=6)分别为100.7%、96.7%、99.4%及102.4%,RSD均小于3.0%。结论建立的HPLC法可同时测定没食子酸、丹参素、原儿茶醛及芍药苷4种成分,方法快速、简便、结果准确,可为心宁片提供更全面、可靠的质量控制方法。 OBJECTIVE To establish a HPLC method for simultaneous determination of gallic acid,tanshinol,protocatechuic aldehyde and paeoniflorin in Xinning tablets. METHODS The samples were separated on a Altima C18( 250 mm× 4.6 mm,5 μm)column,using methanol and 0.15% phosphoric acid( B) as mobile phase at a flow rate of 1.0 m L·min-1. The column temperature was set at 35 ℃. The wavelength was set on 273 nm,283 nm,280 nm and 230 nm. RESULTS The linear ranges of gallic acid was 0.011 2-0.180 2 μg·mL^(-1)( r=1.000 0),tanshinol was 0. 012 5-0. 201 3 μg·mL^(-1)( r=1. 000 0),protocatechuic aldehyde was 0. 001 2-0.020 1 μg·mL^(-1)( r= 1.000 0),paeoniflorin was 0.012 4-0.199 1 μg·mL^(-1)( r=1.000 0),the average recoveries( n=6) were100.7%,96.7%,99.4% and 102.4% respectively,and RSD were less than 3.0%. CONCLUSION A rapid,simple,accurate HPLC method is successfully established for simultaneous determine 4 effective components in Xinning tablets. The method here in described is helpful for the quality control of Xinning tablets.
作者 吴晓冬 苏畅 关潇滢 江玲玲 王淑红 王铁杰 WU Xiaodong;SU Chang;GUAN Xiaoying;JIANG Lingling;WANG Shuhong;WANG Tiejie(Shenzhen Institute for Drug Control,Shenzhen Key Laboratory of Drug Quality Standard Research,Shenzhen,Guangdong 518057,China)
出处 《今日药学》 CAS 2018年第9期595-598,共4页 Pharmacy Today
关键词 心宁片 没食子酸 丹参素 原儿茶醛 芍药苷 质量控制 高效液相色谱法 Xinning tablets gallic acid tanshinol protocatechuic aldehyde paeoniflorin quality control HPLC
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