摘要
目的：分析四君子汤水煎液的成分,探讨药效的物质基础.方法：按照四君子汤处方中药材比例制备水煎液,采用高效液相色谱-质谱法联用,鉴定其水煎液中的有效成分.结果：四君子汤水煎液中检测出10种化学物质,其中皂苷类7种,分别为人参皂苷Rb1、Re、Rg1、Rb2、Rd、Rg3、甘草酸;黄酮类3种,分别为甘草苷、甘草素、甘草香豆素.结论：通过本方法,测定出四君子汤中的10种化学成分,明确了其水煎液的物质基础,其中人参皂苷Rb1、Re、Rg1、甘草酸为四君子汤的指标性成分,为深入研究四君子汤血清药物化学研究打下基础.
Objective Determine the decoction of Sijunzi decoction by HPLC-MS, and to infer the chemical composition in the decoction of Sijunzi decoction. MethodThe method of liquid chromatography and mass spectrometry was used to treat Sijunzi decoction. Result：According to the total ion chromatogram obtained by liquid chromatography and mass spectrometry and the fragmentation peak of mass spectrometry, it was deduced that Sijunzi decoction contained 10 chemical substances, 7 saponins, ginsenoside Rb1, Re, Rg1, Rb2, Rd, Rg3 , glycyrrhizic acid; three kinds of favonoids： liquiritin, glycyrrhizin, licorice coumarin. Conclusion：The ten kinds chemical composition of Sijunzi decoction were determined. It meaned the pharmacodynamics Substance basis of Sijunzi decoction, ginsenosides Rg1、Re、Rb1 and glycyrrhizic acid were the index component.
作者
尚如霞
张碧月
徐卫东
Shang Ruxia;Zhang Biyue;Xu Weidong(Zhen Jiang Food and Drug Supervision and Inspection Center,Zhenjiang Jiangsu 212000;School of Pharmacy,Jiangsu University,Zhenjiang Jiangsu 212013,China)
出处
《现代科学仪器》
2018年第3期65-70,76,共7页
Modern Scientific Instruments
基金
镇江市科技计划资助（社发类重点研发项目）（SH2016020）
