摘要
从安徽镜湖腐烂的蓝藻中筛选出1株细菌,命名为R1,该菌株具有较强的耐镉、溶藻以及可降解微囊藻毒素能力,经16S r DNA序列对比分析鉴定,R1属于气单胞菌属(Aeromonas)。该菌株对Cd^(2+)有很好的耐受性,在含有50 mg/L Cd^(2+)的培养基中仍能正常生长;细菌通过分泌胞外物质溶解铜绿微囊藻,且该胞外物质耐高温,属非核酸或多糖类物质,丙氨酸和苯丙氨酸协同作用而溶藻;在0. 1 mg/L Cd^(2+)存在的情况下,细菌溶藻能力增强;微囊藻毒素的初始浓度为1. 84 mg/L时,7 d时间降解率为40. 2%。
A bacterial strain named as R1 was screened out from rotten blue green algae in Jinghu Lake （Mirror Lake） in Anhui. The strain had the tolerance to cadmium and capable of lysing algae and degradation of microcystins. Through comparative analysis with 16S rDNA sequences, R1 belongs to the genus of Aeromonas . The strain has a good tolerance to Cd 2＋ , and can normally grow in medium containing 50 mg/L of Cd 2＋ , the strain dissolved Microcystis aeruginosa by secreting extracellular substances, and it was well tolerated to high temperature, the substance belongs to non-nucleic acid or polysaccharide, the algae-lysis was synergetic effect of alanine and phenylalanine. In the condition of the existence of 0.1 mg/L of Cd 2＋ , the ability of algae-lysing was enhanced; when initial concentration of microcystins was at 1.84 mg/L, degradation rate of the strain was at 40.2% in seven days.
作者
刘宇
卢存龙
董德武
陆鹏
刘爱民
LIU Yu;LU Cun-long;DONG De-wu;LU Peng;LIU Ai-min(Coll.of Life Sci.,Anhui Normal Uni,Prov.Key Lab.of Biotic Environ＇t ＆ Ecol.Safet;Inst.of Molec.Biol.＆ Biotech.,Wuhui 241000)
出处
《微生物学杂志》
CAS
CSCD
2018年第5期15-20,共6页
Journal of Microbiology
基金
安徽省自然科学基金项目(1408085MC 53)
安徽师范大学2017年度研究生科研创新与实践项目(2017cxsj068)
安徽高校科研平台创新团队资金项目(201549)
皖江城市带退化生态系统的恢复与重建2011协同创新中心基金项目(201507)
关键词
微囊藻毒素降解菌
耐镉
溶藻
microcystins biodegrading bacterium
tolerance to cadmium
algae-lysis
