摘要
目的了解临床上不同尿液的保存方法对尿生化指标的影响,探讨留取24h尿的适宜保存方法。方法收集55例肾脏内科病人的24h尿。按照尿常规白细胞数>200/μL以及细菌数>1000/μL为标准分为感染性尿液样本组(20例)和非感染性尿液样本组(35例)。每例样本留取三份,分别置于防腐剂(二甲苯)、常温(18-28℃)、冷藏(2-8℃)三种不同条件下保存。检测两组在三种保存条件下:(1)尿蛋白:总蛋白(UTP)、微量白蛋白(m Alb/Cr)、尿免疫球蛋白G(IgG)、转铁蛋白(TRF)、α1微球蛋白(α1-MG);(2)尿电解质:钾(K)、钠(Na)、氯(Cl)、钙(Ca)、镁(Mg)、磷(P);(3)尿酸(UA),肌酐(Cr)的含量。比较两组在不同保存条件对尿生化指标的影响。结果两组24h尿液在二甲苯、冷藏、常温三种条件下,尿生化指标测定值均无显著性差异(P>0.05);结论 24h尿液的生化指标的测定不受保存方式的影响。
Objective： To study the effect of different preservation methods on biochemical indexes of urine for exploring the suitable preservation of 24-hour urine. Methods： Totally, 55 samples of 24-hour urine were collected from renal patients. They were divided into infectious sample group（n=20） and non-infectious sample group（n=35）. All samples were then split again, and one was preserved in xylene, one was preserved in room temperature（18-28 degrees celsius）, one was frozen in 2-8 degrees celsius. After 24 hours, the urine protein（ UTP, m Alb/Cr, IgG, TRF,α1-MG）, urinary electrolyte（K, Na, Cl, Ca, Mg, P）, UA, and Cr were compared between two groups for detecting the effect of different preservation on urinary biochemical indexes. Results： The difference of urinary biochemical indexes among the three preservation was significantly unobvious（P〉0.05）. Conclusions： The 24-hour urine is needed to preserved in room temperature, which has a negligible effect on urinary biochemical indexes.
作者
谭光群
石威
黎冬冬
徐华建
李琳彬
Guangqun Tan;Wei Shi;Dongdong Li;Huajian Xu;Binbin Li(Department of Laboratory,Changdu People＇s Hospital,Changdu,Tibet 854000,China;Inspection of Grade 2011,Chongqing Medical University,Chongqing 400016,China;Department of Laboratory,First Affiliated Hospital of Chongqing Medical University,Chongqing 400016,China;Department of Laboratory,Chongqing Traditional Chinese Medicine Hospital,Chongqing 400021,China)
出处
《西藏医药》
2018年第3期8-11,共4页
Tibetan Medicine
关键词
24h尿蛋白
尿生化指标
测定
24-hour Urine
Urinary Biochemical Indexes
Detection
