摘要
建立鸡蛋中苏丹红残留检测的高效液相色谱法,为保障食品安全提供技术支持。样品经正己烷提取,过SPE小柱净化,氮气吹干后乙腈复溶,进行HPLC分析,以Athena C_(18)-WP液相色谱柱4.6×150 mm,5μm为分离柱,乙腈∶水(95∶5,V∶V)为流动相,1.0 mL/min流速等度洗脱,使用紫外检测器,波长为500 nm,柱温为35℃。实验结果表明4种苏丹红在10～1 000μg/kg添加浓度范围内线性关系良好,相关系数R≥0.999 0,较高浓度时回收率范围为62.7%～107.9%,定量下限(LLOQ)规定为添加回收试验中S/N≥10时的最低添加浓度,结果均小于0.5μg/kg。该方法专属性好,简便、准确、快速,适用于禽蛋中苏丹红的检测。
This study has established the determination of Sudan red residue in eggs high-performance liquid chromatography （HPLC）, provide data support to ensure food safety. This experiment samples extracted by n-hexane, SPE column purification, the nitrogen after dry acetonitrile after dissolved, HPLC analysis, an officer using C18-WP liquid chromatographic column 4.6 ×150 mm, 5 microns as the separation column, acetonitrile： water （95∶5, V∶V） as mobile phase, the 1.0 mL/min elution velocity and so on degree, using UV detector, the wavelength of 500 nm, column temperature of 35 ℃ . Experimental results show that the four （including Sudan red in 10 ～ 1000μg/kg add concentration range inside sex is good, the correlation coefficient R of 0.9990 or higher, with high recovery range of 62.7% ～ 107.9%, quantitative limit （LLOQ） regulations for adding recycling experiment S/N of 10 or more add lowest concentration, the results are less than 0.5μg/kg. Good specificity, the method is simple, rapid, accurate and suitable for the detection of Sudan red in the eggs.
作者
苗翠
Miao Cui(Liaoning Quality Inspection and Test Center for Veterinary Drug Feed and Animal Product,Shenyang110016,China)
出处
《现代食品》
2018年第16期124-126,141,共4页
Modern Food
