摘要
目的:优化蒙药瞿麦中总生物碱提取工艺,并建立总生物碱的含量测定方法。方法:采用紫外-可见分光光度法,酸性染料比色法测定总生物碱的含量。结果:对照品溶液浓度在3.27~26.16μg·mL^(-1)时呈良好的线性关系,其回归方程为A=0.0535C-0.0325,r=0.9999(n=5)。测得蒙药瞿麦中总生物碱含量为3.085mg·g^(-1),加样回收率为98.73%,RSD=1.5%(n=9)。结论:本方法操作简便、稳定性好,灵敏度高,重复性好,可作为蒙药瞿麦中总生物碱的含量测定方法。
Objective： To optimize the extraction process of total alkaloids in Mongolian medicine Dianthus su- perbus L. , and to establish the content determination method of total alkaloid~ Methods： Using ultraviolet vis ible spectrophotometric method, acid dye colorimetry is used to determine the content of total alkaloid in Mongo- lian medicine Dianthus superbus L.. Results： The reference substance concentration has good linear relationship in the rang of 3.27-26.16μg·mL^-1 and the regression equation is A=0.0535C--0.0325,r=0.9999（n=9） . The content of total alkaloid is 3.085mg. g 1,the sample recovery rate is 98.38%,RSD=1.7%（n=9）. Con- clusion： The method is convenient,stable,sensitive, which can be used to determine the content of total alkaloid from Mongolian medicine Dianthus superbus L.
作者
王来兵
马岚
白文明
陈建平
郭晓宇
郭慧卿
于姝燕
钮松召
WANG Lai--bing;MA Lan;BAI Wen--ruing(Inner Mongolia Medical University,Hohhot 010059 China)
出处
《疾病监测与控制》
2018年第3期174-176,共3页
Journal of Diseases Monitor and Control
基金
内蒙古医科大学“科技百万”项目(2015YKDKJBW07)
关键词
蒙药瞿麦
响应面优化
总生物碱
含量测定
mongolian medicine dianthus superbus L.
response surface optimization
total alkaloid
content determination
