摘要
建立与优化了基于顶空固相微萃取结合气相色谱-质谱技术（HS-SPME-GC-MS）的彝药＂满山香＂挥发性成分的定性与半定量分析方法。并利用该方法对4个不同产地满山香中挥发性成分进行了分析与评价,共鉴定出了54种成分,云南武定、云南罗平、贵州清镇与湖北神农架产地的样品分别鉴定出51,53,52和50种挥发性成分,其中46种挥发性成分为4个产地共有。采用主成分分析、聚类分析等化学计量学方法比较不同产地满山香挥发性成分的差异。结果表明,通过挥发性组分的分析可以区分4个产地的满山香,而地理位置接近的产地的满山香样品中挥发性成分组成具有一定的相似性。该文所建立的方法稳定可靠,适用于满山香中挥发性成分的快速分析,可以为彝药满山香的质量控制、药物开发和资源的科学利用提供重要依据。
A headspace solid-phase microextraction gas chromatography-mass spectrometry（HS-SPME-GC-MS） method was developed and optimized for qualitative and semi quantitative analysis of volatile components from Schisandra propinqua,a kind of Yi Nationality herb medicine. This method was used for analysis and evaluation of volatile components from S. propinqua from four different geometrical origins（Yunnan Wuding,Yunnan Luoping,Guizhou Qingzhen and Hubei Shennongjia）. 51,53,52,50 compounds were identified from the above four kinds of samples respectively,in which 46 volatile components were contained in all of these four origins.The volatile components in these samples from different geographical origins were then compared by using principal component analysis,cluster analysis and other chemometrics methods. The results suggested that the analysis of volatile components can be used to distinguish the S. propinqua from four origins,and those samples from close geographical origins were more similar in volatile components.The developed method was stable,reliable,and suitable for rapid analysis of volatile components from S. propinqua,providing reference for quality control,drug development and scientific utilization of the herb.
作者
滕中秋
郝庆秀
金艳
郭兰萍
TENG Zhong-qiu;HAO Qing-xiu;JIN Yan;GUO Lan-ping(State Key Laboratory for Infectious Disease Prevention and Control,National Institute for Communicable Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing 102206,China;Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases,Hangzhou 310003,China;State Key Laboratory Breeding Base of Dao-di Herbs,National Resource Center for Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijng 100700,China)
出处
《中国中药杂志》
CAS
CSCD
北大核心
2018年第15期3216-3222,共7页
China Journal of Chinese Materia Medica
基金
国家重点研发计划项目(2017YFC1700701)
国家科技支撑计划项目(2012BAI29B02)
中国中医科学院基本科研业务费自选项目
关键词
满山香
挥发性成分
顶空固相微萃取
气相色谱-质谱联用
不同产地
Schisandra propinqua
volatile components
headspace solid-phase microextraction
gas chromatography-mass spectrometry
different geographical origins
