摘要
目的:探讨IQGAP3在胆管癌中的表达情况及其临床意义。方法:通过RT-PCR和Western blot测定胆管癌组织和细胞系中IQGAP3的表达量,通过平板克隆、CCK8、Ed U增殖实验等来评价IQGAP3在胆管癌细胞增殖中的作用,通过Western blot和磷酸化蛋白芯片验证IQGAP3调控胆管癌细胞增殖中可能存在的相关机制。结果:IQGAP3在胆管癌组织和细胞中均高表达,体外实验表明IQGAP3可以促进胆管癌细胞增殖。结合临床样本数据分析发现IQGAP3的表达和胆管癌患者肿瘤大小、病理分级以及AJCC TNM分期相关,且具有统计学差异(P<0.05)。Kaplan-meier生存分析结果显示高表达IQGAP3的胆管癌患者的生存时间比低表达IQGAP3的胆管癌患者生存时间短。在胆管癌细胞中IQGAP3和WNK1的表达水平呈正相关。结论:IQGAP3在胆管癌中高表达,且可能通过上调WNK1促进胆管癌细胞的增殖。
Objective：To investigate the expression of IQGAP3 in cholangiocarcinoma（CCA） and its clinical significance.Methods：RT-PCR and Western blot were performed to determine the levels of IQGAP3 in CCA tissues and CCA cell lines. Colone formation assay,CCK8 and Ed U proliferation experiment were used to reveal the role of IQGAP3 in CCA cell proliferation in vitro.Western blot and phosphorylated proteinchip array were used to verify the possible mechanisms of IQGAP3 in regulating the proliferation of CCA cells. Results：IQGAP3 was overexpressed in the CCA tissues and cell lines. IQGAP3 promoted the proliferation of CCA cell lines in vitro. The expression of IQGAP3 was significantly correlated with tumor size,pathological grading and AJCC TNM staging in CCA patients combined with clinical sample data. Kaplan-meier survival analysis showed that the survival time of the CCA patients with high IQGAP3 expression was significantly lower than that those with low IQGAP3 expression. The expression level of IQGAP3 was positively correlated with the expression of WNK1. Conclusion：IQGAP3 was overexpressed in CCA and might promote the proliferation of CCA cells by up-regulating WNK1.
作者
邵子诚
张耀东
卢泽法
李相成
Shao Zicheng;Zhang Yaodong;Lu Zefa;Li Xiangcheng(Liver Transplant Center,the First Affiliated Hospital of NMU,Nanjing 210029,China)
出处
《南京医科大学学报（自然科学版）》
CAS
CSCD
北大核心
2018年第8期1043-1048,共6页
Journal of Nanjing Medical University(Natural Sciences)
基金
国家自然科学基金(81670570)
江苏省重点病种规范化诊疗研究基金(BE2016789)
关键词
胆管癌
IQGAP3
增殖
cholangiocarcinoma
IQGAP3
proliferation
