摘要
目的:为了研究糖尿病肾病小鼠的发病机制,寻找预防糖尿病的海洋生物活性物质。方法:通过金枪鱼胰脏酶解液分组喂养db/db小鼠,测定其血清中TC、TG、LDL-C和HDL-C含量,之后采用双向电泳研究db/db糖尿病小鼠的肾脏蛋白。通过差异蛋白筛选、GO功能聚类、代谢通路分析及IPA蛋白相互作用解析,确定金枪鱼胰脏酶解液对db/db小鼠肾脏的调节作用。结论:金枪鱼胰脏酶解液可以降低糖尿病小鼠血清TC、TG、LDL水平,显著提高HDL-C水平。同时胰脏酶解液通过提高磷酸果糖激酶、NADH脱氢酶和ATP合成酶的表达,有效促进糖尿病小鼠的糖代谢和能量代谢,从而达到改善糖尿病临床症状,保护肾脏的作用。
The purpose of this paper is to study the regulation of enzymatic hydrolysate of tuna＇s pancreatic to Ⅱdiabetic mouse（db/db diabetic mouse） kidney and to look for active substance to prevent diabetes. This thesis study the regulation of Tuna pancreatic enzymatic hydrolysate on kidney of type Ⅱ diabetic mice. By animal experiments, db/db mice are fed in different groups. Then determination the content of TC, TG, LDL-C, HDL-C in mouse serum. The regulation of db/db diabetic mouse kidney is studied by two-dimensional electrophoresis. Then detecting differential proteins of db/db mice kidneys, differential protein expression, GO functional analysis and KEGG pathway analysis.Tuna pancreatic hydrolysate can significantly reduce levels of serum TC, TG, LDL, significantly increased HDL-C levels in diabetic mouse. And Tuna pancreatic enzymatic hydrolysate can improve protein expression of Phosphofructokinase,NADH dehydrogenase and ATP synthase. They can promote glucose metabolism and energy metabolism. So as to achieve the effect of improving the diabetic condition.
作者
韩姣姣
李妍妍
崔晨茜
董丽莎
张红燕
司开学
王朝阳
李晔
Choeng Lingzhi
周君
苏秀榕
Han Jiaojiao;Li Yanyan;Cui Chenxi;Dong Lisha;Zhang Hongyan;Si Kaixue;Wang Zhaoyang;Li Ye;Choeng Lingzhi;Zhou Jun;Su Xiurong(School of Marine Science,Ningbo University,Ningbo 315211,Zhejiang;China Medicine University,New Drug Screening Center,Nanjing 210009)
出处
《中国食品学报》
EI
CAS
CSCD
北大核心
2018年第9期33-40,共8页
Journal of Chinese Institute Of Food Science and Technology
基金
海洋经济创新发展区域示范项目(2013710)
宁波市科技局农业与社发重大科技项目(2010C10040)
宁波市教育局重点学科资助项目(szxl1070)
关键词
胰脏酶解液
Ⅱ型糖尿病
双向电泳
差异蛋白
肾病
pancreatic enzymatic hydrolysate
type II diabetes
two-dimensional electrophoresis
kidney differential protein
kidney disease
