摘要
目的了解贵州省东北部地区蚊虫以及蚊传虫媒病毒的种类及其分布。方法 2017年在贵州省德江、江口县村庄的动物饲养圈使用诱蚊灯采集蚊虫,现场分类并冻存于液氮备用。采集的蚊虫标本在实验室处理后接种BHK-21和C6/36细胞分离病毒,同时进行标本中病毒基因检测。使用生物信息学软件对病毒基因组开展分子遗传学分析。结果 2017年在贵州省德江县龙泉乡居池坝村、青龙镇石打头村、江口县快场村共采集蚊虫3属3种7 380只,其中骚扰阿蚊最多,占采集蚊虫总数的61.55%(4 542/7 380),中华按蚊占26.80%(1 978/7 380),三带喙库蚊810只以及其他蚊虫50只。从蚊虫中分离到3株引起组织培养细胞病变的病毒分离物;经分子生物学鉴定,从三带喙库蚊中分离的病毒株GZDJ1765属于Ⅰ型流行性乙型脑炎(乙脑)病毒,另2株病毒株均来自骚扰阿蚊(GZDJ1746-1、GZDJ1752-1),经鉴定为盖塔病毒。3批蚊虫(GZDJ1704、GZDJ1743-2、GZDJ1751-2)乙脑病毒基因PCR检测均为阳性。结论贵州省东北部地区所调查的农村地区动物饲养圈骚扰阿蚊为优势蚊种,蚊虫中携带乙脑病毒和盖塔病毒。
Objective To investigate the distribution patterns of mosquitoes and mosquito-borne viruses in northeastGuizhou province. Methods In 2017, host-seeking mosquitoes were collected from livestock sheds using Ultraviolet lightsin Dejiang and Jiangkou, Guizhou province. The mosquito specimens were classified according to morphology and stored inliquid nitrogen. All the samples were incubated with BHK-21 and C6/36 cells for virus isolation and detected the virus genes. The virus isolates were identified and analyzed by molecular biological methods. Results In total, there were 7 380 mosquitoes from 3 genera and 3 species collected from Dejiang and Jiangkou, Guizhou province, 61.55%（4 542/7 380） ofwhich were Armigeres subalbatus and 26.80%（1 978/7 380） were Anopheles sinensis. Out of the 3 virus strains isolated fromthe mosquitoes, One（GZDJ1765） was identified as type Ⅰ of Japanese encephalitis virus（JEV） isolated from Culex tritaeniorhynchus and two（GZDJ1746-1, GZDJ1752-1） were identified as Getah virus isolated from A. subalbatus. Threemosquito pools（GZDJ1704, GZDJ1743-2, GZDJ1751-2） tested positive for JEV genes y by PCR. Conclusion Armigeres subalbatus was the predominant species in northeast Guizhou. JEV and GETV were isolated.
作者
宋颂
付士红
李元元
李晓龙
王定明
田珍灶
周敬祝
何英
雷雯雯
王环宇
王斌
鲁晓晴
梁国栋
SONG Song;FU Shi-hong;LI Yuan-yuan;LI Xiao-long;WANG Ding-ming;TIAN Zhen-zao;ZHOU Jing-zhu;HE Ying;LEI Wen-wen;WANG Huan-yu;WANG Bin;LU Xiao-qing;LIANG Guo-dong(Public Health Institute of Qingdao University,Qingdao 266071,shandong province,China;State Key Laboratory of Infectious Disease Prevention and Control,National Institute for Viral Disease Control and Prevention,Chinese Center for Disease Control and Prevention;National Institute of Parasitic Diseases,Chinese Center for Disease Control and Prevention,Key Laboratory of Parasite and Vector Biology,Ministry of Health,WHO Collaborating Centre for Tropical Diseases;Guizhou Center for Disease Control and Prevention)
出处
《中国媒介生物学及控制杂志》
CAS
2018年第5期428-435,461,共9页
Chinese Journal of Vector Biology and Control
基金
国家重点研发计划生物安全关键技术研发重点专项(2016YFC1201904)
国家自然科学基金(81290342)~~
关键词
骚扰阿蚊
盖塔病毒
贵州省
Armigeres subalbatus
Getah virus
Guizhou
