摘要
本试验利用低毒、经济的乙醇作为提取液,回流冷凝提取样品中的辣椒素类似物。用高效液相色谱法测定辣椒素、二氢辣椒素和降二氢辣椒素,采用C8色谱柱,粒度5μm,长度150 mm,内径3.9 mm,乙腈-水(体积比40:60)作为流动相,流速1.00 mL/min,在280 nm处进行检测。方法简洁方便,操作简易,可重复性与精密度强,对饲料添加剂辣椒素类似物的检测研究具有很好地指导作用。
In this experiment, the low toxic and economic ethanol was used as the extraction so‐lution, and the capsaicin analogues in the samples were extracted by reflux condensantion. Using high performance liquid chromatography（HPLC） method for the determination of capsaicin analogues,C8 chromatographic column, particle size 5 microns, 150 ㎜ in length, inner diameter 3.9 ㎜, ace‐tonitrile-water（volume ratio 40：60） as mobile phase, flow rate was 1.00 mL/min, at 280 nm. HPLC is simple and convenient, easy to operate, reproducible and high precision, and can guide the de‐tection study of capsaicin analogues in feed additives.
作者
陈玉艳
于家丰
Chen Yuyan;Yu Jiafeng(Liaoning Testing ＆ Inspection Center for Quality ＆ Safety of Veterinary Drugs,Feed and Livestock Products,Liaoning Shenyang 110016)
出处
《现代畜牧兽医》
2018年第10期12-16,共5页
Modern Journal of Animal Husbandry and Veterinary Medicine
关键词
辣椒素类似物
高效液相色谱
饲料添加剂
Capsaicinanalogues
High performance liquid chromatography
Feed additives
