摘要
目的:评价脂肪干细胞(ADSCs)对糖尿病型大鼠勃起功能障碍(ED)的治疗效果,探讨ADSCs治疗ED的机制。方法:108只雄性SD大鼠,其中20只为正常对照,余88只大鼠通过腹腔注射STZ建立糖尿病模型。糖尿病大鼠行阿扑吗啡实验,将实验阴性者分为ED模型组(19只大鼠)和ADSCs治疗组(20只大鼠),前者海绵体移植PBS缓冲液,后者海绵体移植ADSCs悬液。4周后,在10 V刺激下测定海绵体内压(ICP)和平均动脉压(MAP),计算ICP/MAP。采用免疫荧光染色方法检测大鼠阴茎海绵体中v WF、α-SMA、n NOS蛋白的表达。结果:(1)ADSCs增殖能力较强且可被诱导分化为脂肪细胞和成骨细胞。(2)正常组、ED模型组和ADSCs组的ICP/MAP值分别为(0.690±0.096)、(0.250±0.096)和(0.440±0.130),差异有统计学意义(P<0.05),ADSCs组明显高于ED模型组。(3)ADSCs治疗组大鼠阴茎海绵体v WF、α-SMA和n NOS蛋白的表达均高于ED模型组(P<0.05)。结论:ADSCs疗法能够提高大鼠的勃起功能,增加大鼠阴茎海绵体v WF、α-SMA和n NOS蛋白的表达。
Aim：To evaluate the role of adipose tissue-derived stem cell（ ADSCs） in erectile dysfunction（ ED） treatment and explore the mechanism. Methods：A total of 108 male SD rats were enrolled in the experiment,20 were randomly divided into normal control group,and the remaining 88 rats were injected with STZ to induce diabetic model. Rats with diabetic mellitus were tested with APO test,and those with no erection were randomly allocated into ED model group（ n = 19）and ADSCs treatment group（ n = 20）;the former group was intracavernously injected with PBS and the later one with ADSCs suspension. After 4 weeks of intervention,ICP and MAP of the rats were measured and the therapeutic effect was evaluated.The expressions of v WF,α-SMA,and n NOS in corpus cavernosum were detected by immunofluorescence staining. Results：ADSCs had a strong proliferation ability and could be induced to differentiate into adipocytes and osteoblasts. The ICP/MAP of normal control group,ED model group and ADSCs treatment group was（ 0. 690 ± 0. 096）,（ 0. 250 ±0. 096） and（0. 440 ± 0. 130）,and the difference was significant（ P〈0. 05）. ICP/MAP in ADSCs treatment group was significantly higher than that in ED model group. In ADSCs treatment group,the expressions of v WF,α-SMA and n NOS were higher than those in the ED model group（ P〈0. 05）. Conclusion： ADSCs can improve erectile function in rats. ADSCs can enhance the expressions of v WF,α-SMA and n NOS in corpus cavernosum of ED rats.
作者
王文华
葛永超
侯全亮
张程达
葛梦颍
田惠子
任炳楠
岳利敏
杜冰会
李丹康
赵青林
OPOLOT Godfrey
张卫东
WANG Wenhua;GE Yongchao;HOU Quanliang;ZHANG Chengda;GE Mengying;TIAN Hui-zi;REN Bingnan;YUE Limin;DU Binghui;LI Dankang;ZHAO Qinglin;OPOLOT God-frey;ZHANG Weidong(Department of Epidemiology and Statistics,School of Public Health,Zhengzhou University,Zhengzhou 450001;Department of Urology,Zhengzhou Third People＇s Hospital,Zhengzhou 450000;Depart-ment of Internal Medicine,Beaumont Health System,Royal Oak,MI 48073)
出处
《郑州大学学报（医学版）》
CAS
北大核心
2018年第5期675-678,共4页
Journal of Zhengzhou University(Medical Sciences)
基金
河南省科技攻关计划基金资助项目(172102310644)
关键词
脂肪干细胞
糖尿病
勃起功能障碍
大鼠
adipose tissue-derived stem cell
diabetes mellitus
erectile dysfunction
rat
