摘要
目的利用正交设计法优化碱提酸沉法提取朝鲜淫羊藿中总黄酮的工艺条件。方法以NaOH浓度、提取时间、提取次数与料液比为考察因素,以朝鲜淫羊藿总黄酮提取量为考察指标,正交试验优化最佳提取条件。并用紫外分光光度法测定含量。结果最佳工艺条件为:提取溶剂为0. 3%NaOH溶液,回流提取2次,料液比为1∶40(g/m L),时间为2 h,浓缩比例1∶4,盐酸调节pH=3,总黄酮提取量达6. 67%。结论该提取工艺稳定可靠,具有使用价值。
Objective To optimize the extraction conditions of total flavonoids from Epimedium koreanum by alkaliextraction and acid precipitation. Methods Taking NaOH concentration,extraction time,extraction times and ratio ofmaterial to liquid as the factors of investigation,while the extraction amount of total flavonoids from Epimedium korea-num was used as the index,and the optimal extraction conditions were optimized by orthogonal experiment. The contentwas determined by ultraviolet spectrophotometry. Results The optimum conditions were as follows：extraction solventwas 0. 3% NaOH solution,reflux extraction 2 times,ratio of material to liquid was 1∶ 40,time was 2 h,concentrationratio was 1∶ 4,hydrochloric acid was adjusted to pH = 3,and the total flavonoids extraction rate was 6. 67%. Conclu-sion The extraction process is stable and reliable,and has utility value.
作者
刘茂顺
葛乃嘉
苏益平
张艺琼
陆钊
LIU Maoshun;GE Naijia;SU Yiping;ZHANG Yiqiong;LU Zhao(School of Medical Laboratory;School ofPublic Health;College of Pharmacy,Jilin Medical University,Jilin City,Jilin Province,132013,China)
出处
《吉林医药学院学报》
2018年第6期426-429,共4页
Journal of Jilin Medical University
基金
吉林省教育厅"十三五"科学技术项目(JJKH20180826KJ)
关键词
碱提法
正交实验
朝鲜淫羊藿
总黄酮
alkali extraction method
orthogonal design
Epimedium koreanum
totlal flavonoids
