摘要
目的研究服用益生菌VSL#3前后,结肠炎(UC)大鼠肠道菌群的变化以及6种常见细菌毒素基因携带率的改变,同时探究益生菌的最适治疗浓度。方法 5%硫酸葡聚糖钠(DSS)溶液自由饮用一周,构建结肠炎大鼠模型,观察和评价大肠黏膜的组织学损伤,计算DAI评分以及病理学评分。收集各组Sprague-Dawly大鼠(SD大鼠)的粪便标本,通过粪便涂片革兰染色镜检法和细菌培养鉴定进行菌群分析,利用实时荧光定量PCR检测粪便中6种常见细菌的毒素基因。结果益生菌治疗组DAI评分、病理学评分均低于模型组(P<0.05);益生菌治疗组菌群失调程度较DSS造模组减轻(P<0.05),需氧和厌氧条件致病菌的优势生长率均低于DSS组;益生菌治疗后各个小组中多种致病菌毒素基因的总携带率较DSS组降低,但差异不具有统计学意义(P>0.05)。不同浓度益生菌治疗组之间,菌群失调程度,需氧条件致病菌和厌氧条件致病菌的优势生长率,致病菌毒素基因携带率,均无显著性差异(P>0.05),其中中浓度治疗组涂片菌群失调率以及失调程度积分最低。结论益生菌可能通过改善结肠炎大鼠肠道菌群失调情况、降低肠道细菌毒素基因的携带率,从而发挥其对溃疡性结肠炎的治疗作用;中浓度[0.6g/(kg·d)]的益生菌可能是治疗溃疡性结肠炎的最适浓度。
Objective To study changes of the intestinal flora and carriage rate of toxin genes of 6 kinds of common bacteria in mice with ulcerative colitis before and after administering probiotics, and explore the optimal therapeutic concentration ofprobiotics. Methods Those mice were given access to 5% dextran sulfate sodium （DSS） solution ad libitum for a week to build the model of experimental acute enteritis, observe and evaluate the histological damage of large intestine mucosa, and calculate DAI scores and pathological scores. We collected the stool samples of Sprague-Dawly （SD） mice in each group, analyzed the microflora by feces smear with Gram staining microscopic examination and bacterial culture and identification, and detected the toxin genes of 6 kinds of common bacteria in feces with real-time fluorescent quantitative PCR. SPSS 19.0 statistical software package was used for data analysis, and P〈0.05 was considered to be statistically significant. Results DAI scores and pathology scores in probiotics groups were lower than those in the model group （P〈0.05）. In probiotics groups, the disorder degree of microflora was lower than that in DSS group （P〈0.05）, and the dominant growth rate of both aerobic and anaerobic pathogenic bacteria was lower than that in DSS group. The total carriage rate of toxin genes of various pathogenic bacteria in each group was lower than that in DSS group after the treatment with probiotics, but the difference was not statistically significant （P〉0.05）. In groups treated with probiotics of different concentrations, the degree of dysbacteriosis, dominant growth rate of both aerobic and anaerobic pathogenic bacteria, and carriage rate of pathogenic bacterial toxin genes were not significantly different （P〉0.05）, in which the feces smear of concentration treatment group showed lowest degree and rate of dysbacteriosis. Conclusion Probiotics may play a role in the treatment of ulcerative colitis by improving the intestinal flora imbalance in mice with colitis and reducing the carriage rate of bacterial toxin gene in the intestinal tract. Moderate concentration ofprobiotics [0.6g/（kg.d）] may be the optimal concentration for ulcerative colitis.
作者
张孟爽
郑晓者
牛敏
刘淑敏
段勇
杜娜
尧静
杜艳
Zhang Meng-shuang;Zheng Xiao-zhe;Niu Min;Liu Shu-Min;Duan Yong;Du Ya;Yao Jing;Du Yan(Department of Clinical Laboratory,First Affiliated Hospital of Kunming Medical University,Kunming 650032;Yunnan Institute of Laboratory,Kunming 650032;Yunnan Key Laboratory of Laboratory Medicine,Kunming 650032;Department of Genetic Metabolism,The Children＇s Hospital,Zhejiang University School of Medicine,Hangzhou 310052)
出处
《中国抗生素杂志》
CAS
CSCD
2018年第10期1257-1262,共6页
Chinese Journal of Antibiotics
基金
云南省应用基础研究计划(No.2011FZ123)
昆明医科大学应用基础研究联合专项资金面上项目(No.2017FE467(-173))
关键词
溃疡性结肠炎
益生菌
肠道菌群
毒素基因
最佳治疗浓度
Ulcerative colitis
Probiotics
Intestinal flor
Toxin gene
Optimal treatment concentration
