摘要
目的:探讨卵巢在"通调针法"电针治疗乳腺增生(MGH)中的作用,为电针治疗MGH的机制研究提供新的思路。方法:雌性SD大鼠随机分为空白组、模型组、电针组、电针切卵巢组、电针假手术组,每组12只。采用大鼠后肢内侧肌肉注射苯甲酸雌二醇注射液联合黄体酮注射液的方法复制MGH模型。造模成功后,对电针切卵巢组进行双侧卵巢切除术,电针假手术组采取假手术对照。电针组、电针切卵巢组和电针假手术组均采用"通调针法"电针治疗,甲组穴为双侧"天宗""肝俞""足三里",乙组穴为双侧"屋翳""合谷"和"膻中",甲乙两组穴隔日交替使用,20min/次,每日1次,5次为1个疗程,共4个疗程。治疗结束后,测量大鼠乳头高度;ELISA法检测大鼠血清中雌二醇(E2)、黄体酮(P)含量;免疫荧光双染和Western blot法检测乳腺组织中雌激素受体α(ERα)、孕激素受体(PR)的表达。结果:与空白组比较,模型组大鼠乳头高度显著增高(P<0.01),E2、ERα含量增高,P、PR含量下降(P<0.01)。与模型组比较,电针组和电针假手术组大鼠乳头高度减小(P<0.01),而电针切卵巢组改善不明显(P>0.05);电针能够下调电针组和电针假手术组大鼠血清E2及乳腺组织中ERα含量(P<0.01)、上调大鼠血清P和乳腺组织PR含量(P<0.01),电针切卵巢组血清P含量升高(P<0.05),免疫荧光染色法示乳腺组织中ERα降低、PR升高(P<0.01),但两受体改善均较电针组差(P<0.05)。结论:电针治疗MGH的疗效机制与卵巢功能密切相关。
Objective To observe the effect of electroacupuncture（EA）intervention on hyperplastic mammary glands,serum estradiol（E2）and progesterone（P）contents,estrogen receptor alpha（ERα）and progesterone receptor（PR）expression of breast tissues in mammary gland hyperplasia（MGH）rats,so as to reveal its mechanisms underlying improvement of MGH.Methods Sixty female SD rats were randomly divided into control,model,EA,EA＋ovariectomy（OVX）and EA＋sham-OVX groups（n=12 in each）.The MGH model was established by injection of Estradiol Benzoate injection（0.5 mg/kg,once daily for25 d）and P injection（0.5 mg/kg,once daily for 5 dafter estradiol injection）into the medial hind-leg muscle.After model establishment,bilateral OVX was performed for rats of the EA＋OVX group,and sham OVX（only exposure of ovaries）was performed for rats of the EA＋sham OVX group.EA（2 Hz,2 mA）was applied to bilateral＂Tianzong＂（SI 11）,＂Ganshu＂（BL 18）,＂Zusanli＂（ST 36）for point group A,and＂Wuyi＂（ST 15）,＂Hegu＂（LI 4）,＂Danzhong＂（CV 17）for point group B for 20 min,once daily,5 days a week for 4 weeks.The two acupoint groups were used alternately.The height of the rats＇ nipples（the 2 nd pairs）were measured.Serum E2 and P levels were assayed by ELISA.The expression of ERαand PR in mammary tissues was detected by immunofluorescence staining and Western blot,separately.Results The height of nipples,serum E2 content,and mammary ERαprotein expression level were significantly higher in the model group than in the control group（P〈0.01）,while serum P content and PR expression were remarkably lower in the model group than in the control group（P〈0.01）.Following EA intervention,the height of nipples,serum E2 content and mammary ERαprotein expression level in both EA and EA＋sham OVX groups,and ERαexpression in the EA＋OVX group were considerably lower in comparison with those of the model group（P〈0.01）,and serum P content and PR expression in the 3 EA groups were significantly higher than those in the model group（P〈0.01,P〈0.05）.But there were no significant changes in the nipple height and serum E2 levels in the EA＋OVX group relevant to the model group（P〈0.05）,suggesting an important role of the ovary in producing EA＇s effect.Conclusion EA intervention can improve hyperplastic mammary glands by down-regulating serum E2 content and mammary ERαprotein expression,and up-regulating serum P content and PR expression in MGH rats,which has a close relation with the intact ovaries.
作者
郭新荣
张卫华
孟菊星
冯伟
李伟伟
王卫刚
GUO Xin rong;ZHANG Wei-hua;MENG Ju xing;FENG Wei;LI Wei wei;WANG Wei gang(College of Acupuncture-moxibustion and Tuina,Shaanxi University of Traditional Chinese Medicine,Xi＇an 712046,China)
出处
《针刺研究》
CAS
CSCD
北大核心
2018年第10期640-644,共5页
Acupuncture Research
基金
国家自然科学基金面上项目(No.81473782)
陕西省科技厅自然科学基础研究计划面上项目(No.2017JM 8045)
陕西中医药大学自然科学创新基金项目(No.14XJZR 05)
关键词
电针
乳腺增生病
雌二醇
黄体酮
乳腺雌激素受体
孕激素受体
卵巢切除术
Electroacupuncture
Mammary gland hyperplasia
Estradiol （E2）
Progesterone （P）
Mammary E2 and P receptor
Ovariectomy
