摘要
目的初步评价Multi-TestⅡ点刺器在儿童过敏原皮肤点刺试验(SPT)中的适用性。方法选取2017年3～9月就诊的87例过敏性疾病患儿为研究对象,均进行6种吸入性过敏原的SPT和血清特异性IgE(sIgE)测定。分别应用单点刺针及Multi-TestⅡ点刺器于患儿左、右前臂进行SPT的测定,应用荧光酶联免疫法进行血清s IgE浓度的测定。用视觉模拟评分(VAS)标尺评估患儿接受SPT的疼痛程度。比较3种方法 6种过敏原的阳性检出率分布差异及Multi-TestⅡ点刺器的灵敏度和特异度。结果 Multi-TestⅡ法、单点刺针法、荧光酶联免疫法3种方法检出6种过敏原的阳性率比较差异无统计学意义(P>0.05)。与单点刺针法比较,Multi-TestⅡ法灵敏度为72.7%,特异度为78.2%;与荧光酶联免疫法比较,Multi-TestⅡ法灵敏度为73.4%、特异度为77.5%。Multi-TestⅡ法较单点刺针法疼痛程度低,差异有统计学意义(P<0.05)。结论 Multi-TestⅡ法灵敏度及特异度较高且易被患儿接受,但测定结果需同时与患儿的临床病史相结合,必要时结合血清特异性IgE测定结果。
Objective To study the applicability of Multi-Test Ⅱ prick device in the skin prick test（SPT）for allergens in children.Methods A total of 87 children with allergic diseases who attended the hospital between March and September,2017 were enrolled as subjects.The SPT for six inhaled allergens and serum specific IgE（sIgE）measurement were performed for all children.SPT was performed with a single-headed device on the left forearm and Multi-Test Ⅱ prick device on the right forearm.ELISA was used to measure the serum level of sIgE.Visual Analog Scale（VAS）was used to assess the degree of pain during SPT.The three methods were compared in terms of the detection rates of six allergens,and the sensitivity and specificity of Multi-Test Ⅱ prick device were analyzed.Results There were no significant differences in the detection rates of six allergens among the Multi-Test Ⅱ method,the single-headed method,and ELISA（P〈0.05）.Compared with the single-headed method,the Multi-Test Ⅱ method had a sensitivity of 72.7%and a specificity of 78.2%.Compared with ELISA,the Multi-Test Ⅱ method had a sensitivity of 73.4%and a specificity of 77.5%.The Multi-Test Ⅱ method had a significantly lower degree of pain than the single-headed method（P〈0.05）.Conclusions The Multi-Test Ⅱ method has high sensitivity and specificity and is easily accepted by children,but the test results should be combined with children’s medical history and serum level of sIgE when necessary.
作者
王亚如
关辉
王燕
苗青
向莉
WANG YaoRu;GUAN Hui;WANG Yan;MIAO Qing;XIANG Li(Department of Allergy,Beijing Children＇s Hospital Capital Medical University/National Center for Children＇s Health,Beijing 100045,China)
出处
《中国当代儿科杂志》
CAS
CSCD
北大核心
2018年第10期835-839,共5页
Chinese Journal of Contemporary Pediatrics
基金
公益性行业科研专项(2015SQ00136)
北京市自然科学基金资助项目(7172074)
