摘要
建立了固相萃取-超高效液相色谱法同时测定食品中的10种人工合成色素的分析方法。样品经Oasis WAX固相萃取小柱富集和净化,然后经超高效液相色谱仪分析,采用反相Waters ACQUITY UPLC CSH C18柱(2.1 mm×100 mm, 1.7μm)分离,以甲醇-乙酸铵水溶液(0.010 mol/L)为流动相进行梯度洗脱,采用二极管阵列检测器,检测波长范围190～800 nm,在可变波长254 nm下进行检测。结果表明10种人工合成色素线性相关系数良好,相关系数为0.995～1.000,线性范围为0.30～50.0 mg/kg。在1.0, 5.0和10.0 mg/kg三个添加水平下,回收率为86.0%～98.9%,相对标准偏差为0.94%～3.12%,检出限为0.10～0.15 mg/kg。所建立的方法简便,灵敏度高,可以满足对食品中10种人工合成色素的同时测定。
A method for the simultaneous determination of 10 synthetic colours in food by solid phase extraction-ultra high performance liquid chromatography was established. The samples were enriched and purified by Oasis WAX, and then analyzed by UPLC, reverse Cls Accucore column （2.1 mm × 100 mm, 1.7 μm） was used to separate, and methanol ammonium acetate solution （0.010 mol/L） was used as the mobile phase to conduct gradient elution. Ten synthetic colours were detected by a diode army detector, detection wavelength range of 190-800 nm, and detected under variable wavelength of 254 urn. The linear relationship of ten synthetic colours was good with the correlation coefficient of 0.995-1.000 and the linear range was from 0.30 mg/kg to 50.0 mg/kg. The additive recoveries were in the range of 86.0%-98.9% with the relative standard deviation （RSD） of 0.94%-3.12% under the standard addition levels of 1.0, 5.0 and 10.0 mg/kg, and the detected limits often synthetic colours were 0.10-0.15 mg/kg. The method was simple, high sensitivity, and could be used to detect the 10 kinds of synthetic colours in foods at the same time.
作者
黎星
江丰
王会霞
严恒
张小玲
LI Xing, JIANG Feng, WANG Huixia, YAN Heng, ZHANG Xiaoling(Hubei Provincial Institute for Food Supervision and Test,Wuhan 43000)
出处
《食品工业》
CAS
北大核心
2018年第10期325-328,共4页
The Food Industry
关键词
固相萃取
超高效液相色谱
食品
合成色素
solid phase extraction
high performance liquid charomatography
food
synthetic colours
