摘要
废水处理系统被认为是水环境中抗生素抗性基因(ARGs)的重要污染源.为探究ARGs在废水处理系统中的分布特征和去除情况,选取某精细化工园区内的制药废水处理系统和园区综合性废水处理系统,使用PCR和实时荧光定量PCR对不同处理单元中ARGs的存在情况和丰度变化进行研究.在两个系统进水中分别检出了10种和15种ARGs,其中以四环素类和磺胺类ARGs居多,并首次检出了dfrA13基因.进水中sulⅠ和sulⅡ基因的丰度最高,随后依次是dfrA13、tetQ、floR、tetO和tetW基因.制药废水处理系统使总ARGs浓度上升了0.21个数量级,出水汇入园区综合性废水处理系统再次处理,其对综合性废水处理系统进水中总ARGs的贡献率为5.05%.综合性废水处理系统使总ARGs浓度下降了1.03个数量级,残留ARGs同最终出水一起直接排海,对近海环境中微生物群落的潜在影响有待深入研究.
Wastewater treatment plants（ WWTPs） have been regarded as important point-sources of antibiotic resistance genes（ ARGs） in aquatic environments. To investigate the distribution and removal of ARGs in WWTPs,a pharmaceutical wastewater treatment plant（ PWWTP） and an integrated wastewater treatment plant（ IWWTP） in a fine-chemical industrial park were chosen,and polymerase chain reaction（ PCR） and real-time PCR techniques were used to determine the occurrence and abundances of ARGs along the treatment processes. Ten and fifteen ARGs were detected initially in the influents of PWWTP and IWWTP respectively,in which tetracycline and sulfonamide resistance genes were frequently reported,while dfr A13 was first reported in WWTPs. The most abundant ARGs in the influents were sulⅠ and sulⅡ,followed by dfrA13,tetQ,floR,tetO,and tetW. The total ARGs increased by 0. 21 log after the treatment by PWWTP,whose effluent contributed 0. 87% to the inflow yet 5. 05% to the total ARGs of IWWTP. Finally the total ARGs removed by IWWTP was 1. 03 log,with the remaining ARGs then transported within the final effluent to the nearby coastal area. The authors concluded that the environmental and other impacts from the spread of ARGs on the microbial communities of the coastal environment needed further study.
作者
李奥林
陈吕军
张衍
代天娇
田金平
刘锐
温东辉
LI Ao-lin;CHEN Lü-jun;ZHANG Yan;DAI Tian-jiao;TIAN Jin-ping;LIU Rui;WEN Dong-hui(School of Environment,Tsinghua University,Beijing 100084,China;College of Environmental Seienees and Engineering,Peking University,Beijing 100871,China;Zhejiang Provincial Key Laboratory of Water Seienee and Technology,Department of Environmental Teehnology and Eeology,Yangtze Delta Region Institute of Tsinghua University,Jiaxing 314006,China;School of Environment and Civil Engineering,]iangnan University,Wuxi 214122,China)
出处
《环境科学》
EI
CAS
CSCD
北大核心
2018年第10期4593-4600,共8页
Environmental Science
基金
国家自然科学基金项目(51678003,51678334,51708253)
关键词
抗生素抗性基因(ARGs)
废水处理系统
制药
工业园区
定量PCR
antibiotic resistance genes （ARGs）
wastewater treatment plants （WWTPs）
pharmacy
industrial park
real-time PCR （qPCR）
