摘要
目的观察益气活血祛湿解毒膏方对甘露醇所致的急性血栓性浅静脉炎的药效作用并探讨其起效机制。方法实验用健康日本大耳白兔16只随机分为益气活血祛湿解毒膏方组(膏方组)、脉络舒通颗粒组(颗粒组)、空白对照组、模型组,每组4只。釆用甘露醇输液制成血栓性静脉炎兔子模型。膏方组给予益气活血祛湿解毒膏方2. 34 g/kg灌胃,每日2次;颗粒组给予脉络舒通颗粒2. 80 g/kg,每日3次,给药时间7天。釆用酶联免疫法检测血清中TNF-α、IL-6、CRP、IL-1β含量,采用Real Time荧光定量PCR法及Western blot法检测NF-κB、蛋白激酶C(protein kinase C,PKC) m RNA及蛋白表达。结果日本大耳白兔造模后第5~7天陆续出现耳缘静脉红肿,触摸呈条索状,活动减少,食量、饮水减少。与空白对照组比较,模型组TNF-α、CRP、IL-1β、IL-6水平升高,NF-κB、PKC蛋白表达及NF-κB m RNA表达增高(P <0. 05);与模型组比较,颗粒组、膏方组TNF-α、CRP、IL-1β、IL-6水平及NF-κB、PKC蛋白表达降低,且膏方组低于颗粒组(P <0. 05)。结论益气活血祛湿解毒膏方通过降低炎症因子水平,促进炎症恢复。
Objective To observe the pathogenesis effects with Ointment of Tonifying Qi and Activating Blood Eliminate Wetness and Disintoxication（ QTQABEWD） on mannitol infusion phlebitis,and to explore its mechanism. Methods A total of 16 healthy Japanese white rabbits were selected as experimental animal models. The rabbits were randomly divided into the QTQABEWD group（ phlebitis ointment group）,the Mailuo Shutong Granule group（ granule group）,the blank control group and the model group,4 in each group. The thrombotic phlebitis model was established by inducing mannitol firstly except the blank control group. The phlebitis ointment group was treated with QTQABEWD 2. 34 g/kg·d by gavage,twice one day. The rabbits in the granule group was administrated with Mailuo Shutong granules at 2. 80 g/（ kg·d） by gavage,three times one day. The therapy course lasted for 7 days. The levels of tumor necrosis factor-α（ TNF-α）,interleukin-6（ IL-6）,Creactive protein（ CRP）,and interleukin-1（ IL-1）were detected by ELISA. The m RNA and protein expressions of NF-κB p65 and protein kinase C（ PKC） were detec-ted by Real-Time Polymerase Chain Reaction and Western blot. Result The ear of rabbits swelling occurred at 5-7 days after the model was established. Puncture point of peripheral venous was inflamed,swelling,and cord-like,activity and food intake decreasing,and fewer water intake. Compared with the blank control group,the levels of TNF-α,CRP,IL-1β,IL-6,the protein expressions of NF-κB and PKC and the m RNA of expression of NF-κB increased in the model group（ P〈0. 05）. Compared with the model group,the levels of TNF-α,CRP,IL-1β and IL-6 and the protein expressions of NF-κB and PKC were decreased in the phlebitis ointment group and granule group（ P〈0. 05）.The decrease was more obvious in the phlebitis ointment group than in the granule group（ P〈0. 05）.Conclusion The QTQABEWD can reduce the level of inflammatory cytokines and promote inflammation recovery.
作者
李光宗
杨宝钟
庞鹤
李友山
金潇
邢彤
张凡帆
张月
华海琴
LI Guang-zong;YANG Bao-zhong;PANG He;LI You-shan;JIN Xiao;XING Tong;ZHANG Fan-fan;ZHANG Yue;HUA Hai-qin(Department of Peripheral Vascular,Dongfang Hospital of Beijing University of Chinese Medicine,Beijing 100078;Department of Peripheral Vascular,Dongzhimen Hospital of Beijing University of Chinese Medicine,Beijing 100700;Nursing Department,Dongfang Hospital of Beijing University of Chinese Medicine,Beijing 100078;Department of Radiology,Dongfang Hospital of Beijing University of Chinese Medicine,Beijing 100078)
出处
《中国中西医结合杂志》
CAS
CSCD
北大核心
2018年第10期1240-1246,共7页
Chinese Journal of Integrated Traditional and Western Medicine
基金
2010年高校博士点基金项目(No.20100013110001)
北京中医药大学校级课题(No.2015-JYB-JSMS115)
关键词
急性血栓性浅静脉炎
益气活血祛湿解毒膏方
甘露醇
acute thrombotic superficial phlebitis
Ointment of Tonifying Qi and Activating Blood Eliminate Wetness and Disintoxication
mannitol
