摘要
目的建立测定人血清中去甲万古霉素含量的超高效液相色谱法。方法以Waters ACQUITY UPLC BEH C_(18)(2.1 mm×50 mm,1.7μm)为色谱柱,流动相为磷酸二氢钾缓冲液(pH=3.2)-甲醇(88∶12,v/v),流速0.21 mL·min^(-1),柱温42℃,检测波长:236 nm。10%高氯酸沉淀蛋白,离心取上清液进样。结果去甲万古霉素在1.67～83.40μg·mL^(-1)与峰面积线性关系良好(r=0.9991),低、中、高浓度(4.17、25.02、66.72μg·m L^(-1))的提取回收率分别为(66.30±2.1)%、(72.22±1.0)%、(75.32±0.8)%;方法回收率分别为(102.5±5.4)%、(97.5±1.1)%、(101.7±1.8)%。日内及日间精密度均小于3%(n=5)。结论该检测方法操作简单、准确、灵敏,可用于血清中去甲万古霉素的监测。
Objective To establish a UPLC method for norvancomycin concentration detection in the human serum. Methods Norvancomycin was extracted by 10% perchloric acid and separated by a Waters ACQUITY UPLC BEH C_（18）（2.1 mm×50 mm, 1.7 μm） column. The mobile phase was monopotassium phosphate buffer（pH= 3.2）-methyl alcohol（88∶12, v/v）. The flow rate was 0.21 mL·min^-1, the column temperature was at 42℃and the detection wavelength was at 236 nm. Results The linear range was from 1.67 to 83.40 μg·mL^-1, r =0.9991. The extract recovery of low, medium, and high concentrations was（66.30±2.1）%,（72.22±1.0）%, and（75.32±0.8）% while the method recovery was（102.5±5.4）%,（97.5±1.1）%, and（101.7±1.8）%, respectively. The intra-and inter-day RSD was less than 3%（n = 5）. Conclusion This method is simple, accurate, precise and suitable for the monitoring serum concentration of norvancomycin.
作者
李碧艳
何盛泉
LI Bi-yan;HE Sheng-quan(The First Affiliated Hospital of Xiamen University,Xiamen Fujian 361000;Medical College of Xiamen University,Xiamen Fujian 361000)
出处
《中南药学》
CAS
2018年第10期1443-1445,共3页
Central South Pharmacy
