摘要
目的 探讨机体感受和调制痛觉的最高级中枢初级躯体感觉区（S1区）神经元特异性蛋白激酶C γ亚型（protein kinase C γ isoform, PKCγ）在重要功能结构脂筏上转位水平的变化与瑞芬太尼诱导痛觉过敏的联系。 方法 8～10周龄SD大鼠50只，按照随机数字表法分为丙泊酚组（P组，20只）、瑞芬太尼组（R组，20只）和对照组（C组，10只），P组和R组经尾静脉予相应麻醉，C组不予任何措施。P组、R组各取10只大鼠，分别于麻醉前及麻醉停药后0.5、2、5、24 h测定大鼠机械刺激缩足阈值（paw withdrawal mechanical threshold， PWMT）。P组、R组剩余大鼠（各10只）及C组10只大鼠分别于停药后2 h（7只）、停药后24 h（3只）断头处死，分离S1区，Western blot测定全细胞及脂筏PKCγ水平。 结果 ① R组大鼠 PWMT在停止输注后2 h明显低于P组（P〈0.05）。② 停药后2 h，2组动物S1区全细胞PKCγ水平差异无统计学意义（P〉0.05）；R组动物脂筏PKCγ水平明显高于P组（P〈0.05）。 结论 S1区PKCγ脂筏转位增加，证实了瑞芬太尼停药后S1区神经细胞活化。可能是瑞芬太尼诱导痛觉过敏的机制之一，或是瑞芬太尼诱导痛觉过敏后带来的细胞内信号改变。
Objective To determine the involvement of neuron-specific protein kinase C γ isoform（PKCγ） translocation to lipid rafts in primary somatosensory cortex（S1） in remifentanil-induced hyperalgesia. Methods Fifty adult male SD rats weighing 240-260 g （at the age of 8-10 weeks） were randomly assigned to 3 groups： control, the propofol （group P） and the remifentanil group （group R）. Intravenous anesthesia with either propofol （group P） or propofol combined with remifentanil（group R） was performed through a 24 G catheter inserted in tail vein for 3 h. The total doses of propofol were 36.0 mg and 26.0 mg in group P and R, respectively. The dose of remifentanil was 19.5 g in group R. Mechanical allodynia in the rats was evaluated by the von Frey filament test, and PKCγ levels in the whole-cell lysates and lipid rafts in the S1 neurons were determined by Western blot. Results ① The threshold of paw-withdrawal response to von Frey filament stimulation in group R at 2 h after stopping drug infusion was significantly lower than that in group P（P〈0.05）. ② Two hours after stopping drug infusion, the PKCγ protein levels in the lipid rafts of S1 in group R were significantly higher than those in group R（P〈0.05）. Conclusions These results suggest that increased translocation of PKCγ to lipid raft of S1 neurons may contribute to postoperative pain in rats after propofol-remifentanil-based anesthesia.
作者
崔伟华
王珊珊
岳红丽
任艺
韩如泉
李俊发
Cui Weihua;Wang Shanshan;Yue Hongli;Ren Yi;Hart Ruquan;Li Junfa(Department of Anesthesialogy,Beijing Tiantan Hospital,Capital Medical University,Beijing 100050,China;Department of Neurobialogy,Capital Medical University,Beijing 100069,China)
出处
《国际麻醉学与复苏杂志》
CAS
2018年第10期930-933,955,共5页
International Journal of Anesthesiology and Resuscitation
基金
首都医科大学基础-临床合作科研项目（16JL51）
北京市自然科学基金（7173255）
关键词
瑞芬太尼
痛觉过敏
蛋白激酶Cγ亚型
脂筏
初级躯体感觉区
Remifentanil
Hyperalgesia
Protein kinase C γ isoform
Lipid rafts
Primary somatosensory cortex
