摘要
目的 :对应用细菌内毒素检测试剂盒(终点显色基质法)(以下简称为"试剂盒")检测细菌内毒素中的不同血清样本处理方法进行比较。方法 :在10名健康志愿者的血清中外加细菌内毒素,然后分别采用高氯酸法、新高氯酸法、碱试剂法和高温稀释法进行处理,最后用试剂盒检测,计算细菌内毒素回收率并进行统计学分析。结果 :4种血清样本处理方法的细菌内毒素回收率均在一般鲎试验检测法要求的范围内(50%～200%),但它们在同种外加细菌内毒素浓度下的回收率间均存在显著差异(均P <0.001)。对含不同外加细菌内毒素浓度的血清样本,除碱试剂法的回收率间没有显著差异外,其余3种处理方法的回收率间也均存在显著差异(均P </=0.001)。结论 :对试剂盒检测,选用碱试剂法处理血清样本的细菌内毒素回收率更适宜且稳定性更好,检测细菌内毒素的准确性更高。碱试剂法应用作试剂盒检测的首选血清样本处理方法。
Objective： To evaluate the different methods for the treatment of serum samples with clinical bacterial endotoxin test kit （end-point chromogenic method） （hereinafter referred to as ＂test kit＂）. Methods： Endotoxin was added into the serum samples from ten healflly people and then the samples were treated by different mefllods such as perchloric acid and its improved type, alkali reagent and high-temperature dilution, respectively. The recovery of endotoxin was calculated and statistically analyzed after determination with the test kit. Results： The spike recovery of endotoxin in the samples treated with different methods were within the range required by the general limulus test method （50%-200%）. However, there were significant differences in the spike recoveries when the same concentrations of spiked endotoxin were added into the samples treated by different methods （P〈0.001）. There were also significant differences in the spike recoveries when different concentrations of spiked endotoxin were added into the samples treated with the methods other than alkali reagent （P〈/=0.001）. Conclusion： The spike recovery of endotoxin is accuracy and consistency in the samples treated with alkali reagent, which can be considered as the first choice method for serum sample treatment with the test kit.
作者
陈晓佳
李红玲
林巧辉
丁友玲
CHEN Xiaojia;LI Hongling;LIN Qiaohui;DING Youling(Fuzhou Xinbei Biochemical Industrial Co.,Ltd.,Fuzhou 350101,China)
出处
《上海医药》
CAS
2018年第21期7-11,15,共6页
Shanghai Medical & Pharmaceutical Journal
关键词
细菌内毒素
终点显色基质法
鲎试剂
血清处理方法
回收率
bacterial endotoxin
end-point chromogenic
Tachypleus amebocyte lysate
serum treatment method
spike recovery
