摘要
【目的】筛选优质生防芽孢杆菌资源,开展生防应用研究。【方法】从高产桃园土壤中分离、筛选具有生防作用的细菌菌株,并对其进行形态学、16SrDNA和生理生化鉴定。分别利用鲜桃果实、桃树叶片和枝条进行抑制实验,结合扫描和透射电镜观察。【结果】获得桃褐腐拮抗细菌菌株B-23,抑菌率为73.68%。经鉴定菌株B-23为特基拉芽孢杆菌(Bacillus tequilensis)。以1×10~9 CFU/mL B-23菌悬液处理桃果实、叶片和枝条,对桃褐腐病均具有很好的抑制作用,抑菌率分别为74.48%、83.33%和60%。受抑制的桃褐腐病菌株,在光学显微镜下可观察到菌丝顶端膨大,孢子边缘干瘪;扫描电镜观察发现菌丝表面粗糙,有碎屑产生,孢子表面粗糙且干瘪皱缩;通过透射电镜发现细胞壁降解,细胞器消失,液泡变形。【结论】本试验获得优质桃褐腐病生防菌株,离体试验显示具有良好抑制效果,具有进一步开发前景。
【Objective】The resources of bacillus were screened and the application research was carried out. 【Methods】 In this experiment, biocontrol bacteria were isolated and identified from the soil of high yield peach orchard, by morphology, 16S rDNA sequencing analysis and biochemical and physiological were identified. The inhibition experiments were carried out by fresh peach fruit, peach tree leaves and shoots in vitro , the antibacterial effect and possible mechanism were clarified through scanning and transmission electron microscopy.【Results】The antagonistic bacteria strain B-23 was obtained, the inhibition rate was 73.68%, the strain was identified as Bacillus tequilensis . The fruits, leaves, and shoots of peach trees were treated by 1×10 9 CFU/mL B-23 strain suspension, which had a good inhibitory effect on peach brown rot, the inhibition rate was 74.48%, 83.33% and 60% respectively. After the peach brown rot was inhibited by the antagonistic bacteria, results showed that the mycelium was swelled in apex and the edge of spores was wizened by optical microscopy （OM）; the mycelia were obviously attenuated, with rough surface, most spores were shriveled under scanning electron microscopy （SEM）; In cells of the pathogen inhibited by the antagonistic bacteria, the cell wall was degradation, the organelles were disappeared and the intracellular vacuolar were deformed. The experimental results showed one good inhibitory strain was obtained and its has obvious inhibitory effect, further research should be done.
作者
袁雪
侯旭
胡晓
常昊天
杨瑞
刘悦萍
YUAN Xue;HOU Xu;HU Xiao;CHANG Haotian;YANG Rui;LIU Yueping(a.Key Laboratory of Urban Agriculture（North China）,Ministry of Agriculture,Beijing University of Agriculture,College of Biological Sciences Engineering;1b.Beijing University of Agriculture,Plant Science and Technology College,Beijing 102206,China;2.Beijing University of Agriculture,Introduction Beijing Key Laboratory for Agricultural Application and New Technique,Beijing 102206,China)
出处
《北京农学院学报》
2018年第4期7-13,共7页
Journal of Beijing University of Agriculture
基金
2015 “菜篮子”新型生产经营主体科技能力提升工程
关键词
桃褐腐病
鉴定
特基拉芽孢杆菌
生物防治
peach brown rot
identification
Bacillus tequilensis
biocontrol
