摘要
目的:筛选医疗机构制剂活血益气方抗瘤丸(简称KLW)的制备工艺。方法:将足够量的大鼠神经胶质瘤C6细胞注射至裸鼠右侧腋窝,建立裸鼠荷瘤模型,按瘤块体积随机分组,分别灌胃给予等量的不同工艺KLW1～4,以替莫唑胺为阳性对照,以溶媒(去离子水)为模型对照,测量裸鼠体重、瘤体积,并观察给药前后小鼠症状;连续给药15 d后,处死,称量裸鼠瘤重量。结果:KLW-1中、高剂量组裸鼠瘤块重量,KLW-1高剂量组瘤体积,均低于模型对照组,差异有统计学意义(P <0. 05),阳性对照组瘤块重量及瘤体积均低于模型对照组,差异有统计学意义(P <0. 01)。其他处方组裸鼠瘤块重量、体积与模型对照组比较,差异无统计学意义(P> 0. 05)。各待测组(KLW-1～4高、中、低剂量组)裸鼠体重与模型对照组比较,差异均无统计学意义(均P> 0. 05),阳性对照组给药后裸鼠体重明显低于模型对照组,差异有统计学意义(P <0. 01)。各组有少量裸鼠死亡。结论:抗瘤丸在荷瘤裸鼠模型中具有一定抑瘤效果,与待测工艺比较,原制剂工艺抑瘤效果较好,成本低廉,适用于大规模现代化生产。
Objective To screen the preparation technology of qi reinforcement and blood circulation promotion prescription-Kang Liu Wan （KLW） for medical institutes. Methods： A sufficient amount of C6 mice neuroglioma cell were injected into nude mice right armpit to establish a Nude Mice Tumor-Bearing model.Experimental mice were randomly divided into groups based on model tumor size and intragastrically administered with equal amounts of KLW1-4.Temozolomide was set as a positive control and vehicle （deionized water） as a model control.The nude mice weight and tumor size were measured,and the symptoms of mice was observed during the whole experiment.After 15 day′s experiment,the tumor was weighed. Results： The tumor weight of KLW-1 middle and high dose group were lower than those in the model control group,and the difference was statistically significant （ P 〈0.05）.The tumor weight and tumor size in the positive group were lower than those in the model control group,and the difference was statistically significant （ P 〈0.01）.Compared with control group,all other prescription groups had no significant difference in the weight and size of tumor,and the difference was not statistically significant （ P 〉0.05）.And all preparation technology test groups had no statistically significant difference in weight （ P 〉0.05） when compared with control group.The positive control group was significantly decreased when compared with control group after treatment,and the difference was statistically significant （ P 〈0.01）.A small number of nude mice died in each group. Conclusion： KLW has a specific anti-tumor effect in Nude Mice Tumor-Bearing model.Compared with preparation test technology,traditional prescription process technology has advantage in anti-tumor effect,prescription cost and mass-production technologies adaptability.
作者
陈菲
冯英楠
张鹏
庄伟
林晓兰
Chen Fei;Feng Yingnan;Zhang Peng;Zhuang Wei;Lin Xiaolan(Department of Pharmacy,Xuanwu Hospital,Capital Medical University,Beijing 100053,China)
出处
《世界中医药》
CAS
2018年第10期2600-2603,共4页
World Chinese Medicine
基金
北京市科委十病十药(Z141100002214016)
北京市教委2017年度首都中医药研究专项(1ZY13)
2016年北京市中医药科技发展资金项目(JJ2016-10)
关键词
抗瘤丸
神经胶质瘤
裸鼠
抑瘤
工艺
Kang Liu Wan
Neuroglioma
Nude mice
Anti-tumor
Preparation technology
