摘要
目的：通过检测Thl型细胞因子IL-12和Th2型细胞因子IL-10的含量,来评价慢性束缚（S）联合番泻叶（F）灌胃法复制的D-IBS肝郁脾虚型病证结合模型。方法：将40只SPF级Wistar大鼠（雌雄各半）,随机平均分为空白组及D-IBS＂肝郁脾虚证＂模型Ⅰ（造模7 d）组、Ⅱ（造模14d）组、Ⅲ（造模21 d）组。除空白组灌服等体积的纯净水外,其余各模型组采用S＋F复制D-IBS肝郁脾虚型病证结合大鼠,分别于造模第7、14、21天采用酶联免疫吸附测定法（ELISA）检测血清1L-12、1L-10含量。结果：与空白组比较,D-IBS肝郁脾虚证模模型Ⅰ、Ⅱ、Ⅲ组Th1促炎因子IL-12的含量显著升高（P〈0.05,P〈0.01）,模型Ⅰ、Ⅱ、Ⅲ组Th2抑炎因子IL-10的含量显著降低（P〈0.01）。结论：S＋F灌胃法复建的D-IBS＂肝郁脾虚证＂模型可能存在Th1/Th2平衡漂移,是一种较好的研究中医药治疗D-IBS疗效机制的动物模型。
Objective：To assess diarrhea-predominant irritable bowel syndrome（D-IBS） model rats with liver stagnation and spleen deficiency syndrome（GYPDS） by chronic restraint and senna gavage through contents of Th1 cytokine interleukin（IL）-12 and Th2 cytokine interleukin（IL）-10.Methods： We randomly divided 40 Wistar rats of SPF grade（half males and half females） into normal control group, and D-IBS model with GYPDS modelⅠ（7 d）,Ⅱ（14 d）,Ⅲ（21 d） groups,with 10 in each group. Rats in normal control group were gavaged with equal volume of purified water, while those D-IBS model groups with GYPDS by chronic restraint and senna gavage for 7,14 and 21 days. Then we detected the contents of IL-12 and IL-10 in serum and intestinal mucosa in each group.Results： Compared with those in normal control group, IL-12 levels in serum were increasedobviously in D-IBS model with GYPDS modelⅠ,Ⅱ and Ⅲgroups（P〈0.05,P〈0.01）. IL-10 levels in serum were decreased obviously in model Ⅰ,Ⅱ and Ⅲgroups（P〈0.01）.Conclusion： The shift of Thl/Th2 balance in D-IBS model with GYPDS by chronic restraint and gavagemight be a better animal model used for studying D-IBS by Chinese medicine.
作者
郭军雄
许小敏
刘雨娟
马丽
汪斌
GUO Junxiong;XU Xiaomin;LIU Yujuan;MA Li;WANG Bin(Medicine College of Hexi University,Zhangye 734000,Gangsu,China;Silk Road Traditional Chinese Medicine Research Center,Hexi University,Zhangye 734000,Gangsu,China)
出处
《中华中医药学刊》
CAS
北大核心
2018年第11期2590-2592,共3页
Chinese Archives of Traditional Chinese Medicine
基金
国家自然科学基金地区基金项目(81660759)
关键词
肠易激综合征
腹泻型
肝郁脾虚证
动物模型
irritable bowel syndrome
diarrhea - predominan
liver stagnation and spleen deficiency syndrome
ani-mal model
