摘要
以荷花‘微山湖红莲’实生苗为试验材料,研究镉(Cd,50μmol·L^(-1))胁迫下,外源乙烯前体1-氨基环丙烷羧酸(ACC,100μmol·L^(-1))、ACC与一氧化氮合酶(NOS)抑制剂N-硝基-L-精氨酸(L-NNA,200μmol·L^(-1))、ACC与硝酸还原酶(NR)抑制剂钨酸钠(Tu,1mmol·L^(-1)),ACC与一氧化氮(NO)清除剂2-苯基-4,4,5,5-四甲基咪唑啉-3-氧代^(-1)-氧(PTIO,200μmol·L^(-1)),外源NO供体硝普钠(SNP,500μmol·L^(-1))、SNP与乙烯信号转导抑制剂硫代硫酸银(STS,100μmol·L^(-1))处理下荷花幼苗叶片的受害程度及抗坏血酸(AsA)-谷胱甘肽(GSH)循环的变化情况.结果表明:Cd胁迫下,荷花叶片受害症状明显,其相对电导率、丙二醛(MDA)、AsA和GSH含量显著上升,抗坏血酸过氧化物酶(APX)、谷胱甘肽还原酶(GR)、单脱氢抗坏血酸还原酶(MDHAR)和脱氢抗坏血酸还原酶(DHAR)活性明显降低;ACC的添加进一步增加了Cd对荷花叶片的毒害症状,并加剧了4种抗氧化酶活性的降低,但增加了抗氧化剂的含量; SNP的添加对荷花叶片的伤害起到加重作用,并导致GR和MDHAR活性降低以及AsA和GSH含量的升高; PTIO可显著提高Cd和ACC复合处理下荷花叶片APX、GR、MDHAR和DHAR的活性并降低AsA和GSH的含量,而L-NNA和Tu效果不如PTIO明显; STS可显著缓解Cd和SNP复合处理下荷花叶片的毒害症状,并提高4种抗氧化酶的活性、降低AsA和GSH的含量.由此说明,乙烯和NO在AsA-GSH循环中存在互作,二者相互促进,共同调控AsA-GSH循环,进而参与调控荷花对Cd胁迫的响应.
Under the background of Cd（ 50 μmol·L^-1） stress,we added ethylene precursor ACC（ 100 μmol·L^-1）,ACC ＋ nitric oxide synthase（ NOS） inhibitor L-NNA（ 200 μmol·L^-1）,ACC＋ nitrate reductase（ NR） inhibitor Tu（ 1 mmol·L^-1）,ACC ＋ nitric oxide（ NO） scavenger PTIO（ 200 μmol·L^-1）,NO donor SNP（ 500 μmol·L^-1）,SNP ＋ ethylene signal inhibitor STS（100μmol·L^-1） to examine their effects on the damage degree of leaves and response mechanisms of AsA-GSH cycle in lotus ‘Weishanhuhonglian＇. Results showed toxic symptom of lotus leaves under Cd stress. The relative conductivity,malondialdehyde（ MDA）,as well as ascorbic acid（ AsA） and glutathione（ GSH） contents were significantly increased,but the activities of ascorbate peroxidase（ APX）,glutathione reductase（ GR）,monodehydroascorbate reductase（ MDHAR） and dehydroascorbate reductase（ DHAR） were obviously decreased. Compared with Cd stress,adding ACC significantly increased the damage area of lotus leaves,decreased activities of the above-mentioned four antioxidant enzymes and increased AsA and GSH contents. SNP aggravated the toxic symptom of lotus leaves and decreased GR and MDHAR activities. PTIO significantly relieved the toxic symptom of leaves,increased activities of APX,GR,MDHAR and DHAR,but decreased AsA and GSH contents compared with Cd and ACC treatment. However,the effects of L-NNA and Tu were not as obvious as PTIO＇s. In comparison with Cd and SNP treatment,STS relieved the toxic symptom of leaves,increased APX,GR,MDHAR and DHAR activities,and decreased AsA and GSH contents. Taken together,these results showed the synergistic effects of ethylene and NO in regulating lotus responses to Cd stress through AsA-GSH cycle.
作者
袁满
徐迎春
牛叶青
周慧
安奕霖
金奇江
王彦杰
YUAN Man;XU Ying-chun;NIU Ye-qing;ZHOU Hui;AN Yi-lin;JIN Qi-jiang;WANG Yan-jie(College of Horticulture,Nanfing Agricultural University,Nanjing 210095,China)
出处
《应用生态学报》
CAS
CSCD
北大核心
2018年第10期3433-3440,共8页
Chinese Journal of Applied Ecology
基金
中央高校基本科研业务费专项资金项目(KJQN201546)
国家自然科学基金项目(31400600
31772346)资助~~
