摘要
为探讨狼尾草属优质牧草的遗传多样性,利用SRAP分子标记方法,对13份狼尾草属优质牧草种质进行遗传多样性分析,并构建了DNA指纹图谱,结果表明:(1)从72对SRAP引物中筛选出16对引物进行PCR扩增,共扩增出278条带,其中有218条具有多态性,平均多态率为78.42%。(2)Shannon信息指数(I)和Nei's基因多样性指数(H)平均值分别为0.5017、0.3306,说明13份供试品种(系)具有较为丰富的遗传多样性。(3)利用UPGMA构建13份狼尾草种质资源的聚类图,在遗传相似系数为0.86处可将13份狼尾草品种(系)分为5类。(4)13个狼尾草品种(系)间存在较大的遗传差异,遗传距离在0.0206～0.9740之间。(5)利用1对引物扩增的17个多态性位点构建了狼尾草13个品种(系)的DNA指纹图谱,能有效区分13个品种(系)。
In order to understand the genetic diversity of Pennisetum Rich, the genetic diversity of 13 Pennisetum was analyzed based on SRAP markers, and the DNA fingerprints among them were constructed.The results showed that：（1）16 pair of primers were screened from 72 SRAP primers and 278 SRAP bands were obtained, including 218 polymorphic bands, with a polymorphism rate of 78.42%.（2）The average value of Shannon’s information index（I）and Nei’s gene diversity（H）were 0.5017, 0.3306, respectively,which indicated that there was comparatively great genetic diversity among 13 Pennisetum cultivars.（3）The cluster analysis conducted with UPGMA showed that 13 Pennisetum cultivars were divided into five groups when the genetic similarity coefficient was 0.86.（4）The genetic distance was 0.0206-0.974 between 13 Pennisetum indicating that large genetic differences existed among 13 Pennisetum cultivars.（5）The DNA fingerprints of 13 Pennisetum cultivars were established with 17 sites from one pair of primers, and 13 Pennisetum cultivars could be identified by SRAP fingerprints.
作者
石秀兰
陈平
于得水
韩瑞宏
刘萍
SHI Xiu-lan;CHEN Ping;YU De-shui;HAN Rui-hong;LIU Ping(College of Horticulture and Landscape,Zhongkai University of Agriculture and Engineering,Guangzhou 510225,China;Pratacultural College,Gansu Agricultural University,Lanzhou 730070,China)
出处
《广东农业科学》
CAS
2018年第10期55-60,共6页
Guangdong Agricultural Sciences
基金
广东省科技计划项目(2015A0202091
2016A020207003)
广州市科技计划项目(穗科创字[2016]172号)
广东省土地复垦植被恢复工程技术研究中心建设项目(粤科函政字[2013]1589号)
