摘要
为了解侧柏在镉胁迫下的生理响应,本研究采用水培方式,设置不同梯度CdCl2浓度(50、100、200、300、400和500μmmol/L),对侧柏幼苗进行处理,并进行相关生理生化指标测定。结果表明:随着CdCl2浓度提高,侧柏幼苗中的叶绿素含量呈先降后升趋势;经CdCl2处理的侧柏叶片超氧化物歧化酶活性和可溶性糖含量显著提高,可溶性蛋白含量则有所降低,而谷胱甘肽含量波动变化,其中CdCl2浓度为200μmmol/L时,侧柏各生理指标较其它胁迫浓度均较低。研究发现,侧柏能通过提高渗透调节物质含量和增加清除活性氧自由基的相关酶或非酶物质累积,来应对一定浓度镉胁迫,并产生一定程度适应性。
In order to explore the physiological response of Platycladus orientalis under cadmium stress, different levels of CdCl2 concentrations （50, 100,200, 300,400 and 500μmmol/L） are set up by water culture method to treat P. Orientalis seedlings in this study, and relevant physiological and biochemical indexes are determined. The results show that （ 1 ） the chlorophyll content in the leaves of P. Orientalis showed a trend of fall after rise. （2） The activity of superoxide dismutase and soluble sugar content is significantly increased, while the soluble protein content decreases significantly. When CdCl2 concentration is 200μmmol/L, the physiological indexes of P. Orientalis are lower than other stress concentrations. The above resuhs show that P. Orientalis can adapt to cadmium stress at a certain concentration by increasing the content of osmotic regulatory substances and the accumulation of enzymes or non - enzymatic substances related to scavenging free radicals of reactive oxygen species.
作者
姚侠妹
胡欣
偶春
韩金怡
王伟
YAO Xia-mei;HU Xin;OU Chun;HAN Jin-yi;WANG Wei(School of Biology and Food Engineering,Fuyang Normal College,Fuyang 236037,China;Engineering Technology Research Center of Anti-aging Chinese Herbal Medicine,Fuyang Normal College,Fuyang 236037,China;State Key Laboratory Breeding Base Sustainable Pest and Disease Control of Zhejiang,Zhejiang Academy of Agricuhural Sciences,Hangzhou 310021,China)
出处
《榆林学院学报》
2018年第6期71-74,共4页
Journal of Yulin University
基金
安徽省高校自然科学研究重点项目(KJ2018A0349)
浙江省植物有害生物防控重点实验室开放基金项目(2010DS700124-KF1816)
阜阳市政府-阜阳师范学院横向合作一般项目(XDHX201713)
阜阳师范学院自然科学研究重点项目(2018FSKJ03ZD)
阜阳师范学院青年人才基金重点项目(rcxm201806)
国家级大学生创新训练项目(201810371038)
安徽省大学生创新训练项目(201710371068)
关键词
侧柏
镉胁迫
生理生化指标
Platycladus Orientalis
cadmium stress
physiological and biochemical indexes
