摘要
以沙芥属植物沙芥和斧形沙芥幼苗为试材,采用盆栽控水干旱方法,分析其在不同干旱胁迫强度下根和叶的活性氧水平、抗氧化酶活性和抗氧化剂含量的变化,并利用隶属函数法和抗旱系数法综合评价沙芥和斧形沙芥的抗旱性。结果表明:(1)随着干旱胁迫程度的加剧,沙芥和斧形沙芥的根和叶中O-·2产生速率及·OH、H2O2、MDA含量总体呈逐渐升高的趋势,且干旱胁迫下沙芥比斧形沙芥产生了更多的ROS和MDA。(2)随着干旱胁迫程度的加剧,沙芥和斧形沙芥的根和叶中POD、APX、GST活性及叶中GR活性均先升高后降低,叶中的SOD活性以及根中GR、GPX活性均先降低后升高,根和叶中的CAT活性、叶中的GPX活性和根中SOD活性均逐渐升高;但根和叶中的SOD、POD、CAT活性在各干旱处理下均表现为斧形沙芥高于沙芥。(3)沙芥和斧形沙芥的根和叶中AsA含量随着干旱胁迫程度的加剧而先升高后降低,GSH含量逐渐升高,CAR含量逐渐降低,而VE含量在叶中逐渐升高,在根中却逐渐降低;但斧形沙芥比沙芥合成更多的AsA和GSH,其植物体内AsA-GSH循环系统能清除更多的ROS。(4)沙芥和斧形沙芥的根和叶中总抗氧化能力(T-AOC)均随着干旱加剧逐渐增强,且斧形沙芥的总抗氧化能力强于沙芥;活性氧清除系统的平均隶属度和综合抗旱系数显示,轻度干旱胁迫下沙芥抗旱性强于斧形沙芥,中度和重度干旱胁迫下斧形沙芥的抗旱性强于沙芥。研究认为,在干旱胁迫条件下,斧形沙芥根叶中ROS和MDA含量明显低于沙芥,而其大部分抗氧化酶活性和抗氧化剂含量高于沙芥,斧形沙芥植株体内抗氧化系统表现出更强的活性氧清除能力,从而表现出更强的抗旱性。
The changes of reactive oxygen species,antioxidant enzyme activity,and antioxidant substance contents in roots and leaves of Pugionium cornutum（L.）Gaertn.and P.dolabratum Maxim.were measured with pot-culture drought stress in greenhouse,and the drought resistance of them were evaluated by the method of membership function and drought resistance coefficient.The results showed that：（1）with the increase of drought stress,the O-·2 generation rate and the contents of·OH,H2 O2 and MDA increased in roots and leaves of P.cornutumand P.dolabratum.Moreover,P.cornutumproduced more ROS and MDA than that of P.dolabratumunder drought stress.（2）With the increase of drought stress,the activities of POD,APX and GST increased firstly and then decreased,the activity of CAT increased in roots and leaves;The activity of SOD in leaves,GR and GPX in roots decreased firstly and then increased,theactivity of GR in leaves increased firstly and then decreased,but the activity of GPX in leaves,SOD in roots increased;The activities of SOD,POD and CAT in roots and leaves of P.dolabratum were higher than that in P.cornutum.（3）The content of AsA increased firstly and then decreased,the content of GSH increased,and the content of CAR decreased in roots and leaves;The content of VE in leaves increased,the content of VE in roots decreased;P.dolabratum made more AsA and GSH,the AsA-GSH cycle system in P.dolabratumcould remove more ROS than that in P.cornutum.（4）The T-AOC（total anti-oxidant capacity）increased in roots and leaves;The antioxidant ability of P.dolabratum was higher than that of P.cornutum.We know that drought resistance of P.cornutum were higher than that of P.dolabratumunder light drought stress.However,drought resistance of P.dolabratum was higher than that of P.cornutumunder moderate and severe drought stress by comprehensive drought resistance coefficient and average subordinate function of reactive oxygen species scavenging system indexes in response to drought stress of two species in Pugionium Gaertn.Under drought stress,the ROS and MDA contents in P.dolabratum were lower than those in P.cornutum,and the most of antioxidant enzyme activities and antioxidant contents in P.dolabratum were higher than those in P.cornutum.The reactive oxygen species scavenging system showed stronger drought resistance in P.dolabratumthan those in P.cornutum.
作者
郑清岭
杨忠仁
张凤兰
张晓艳
郝丽珍
ZHENG Qingling;YANG Zhongren;ZHANG Fenglan;ZHANG Xiaoyan;HAO Lizhen(Agricultural College,Inner Mongolia Agricultural University,Inner Mongolia Key Laboratory of Wild Peculiar Vegetable Germplasm Resource and Germplasm Enhancement,Huhhot 010019,China)
出处
《西北植物学报》
CAS
CSCD
北大核心
2018年第9期1674-1682,共9页
Acta Botanica Boreali-Occidentalia Sinica
基金
国家自然科学基金(31760570
31101541
31160393)
公益性行业(农业)科研专项(201203004)
关键词
沙芥
斧形沙芥
干旱胁迫
活性氧清除系统
抗旱性
Pugionium cornutum （L.） Gaertn.
Pugionium dolabratum Maxim.
drought stress
reactive oxygen species scavenging system
drought resistance
