摘要
以紫花含笑(Michelia crassipes)为母本,含笑(M. figo)、深山含笑(M. maudiae)和阔瓣含笑(M. platypetala)为父本,研究其杂交亲和性。采用离体培养法对父本花粉生活力进行检测,利用荧光显微镜观察不同杂交组合花粉萌发和花粉管伸长情况,并调查种间杂交坐果率和杂种萌发特性。结果表明:紫花含笑、含笑和深山含笑的花粉萌发率达65%以上,而阔瓣含笑的花粉萌发率较低,为29.07%。荧光显微观察发现,紫花含笑×含笑、紫花含笑×深山含笑的花粉萌发率和花粉管生长速度比紫花含笑自交快,且花粉管能正常伸长完成授精;而紫花含笑×阔瓣含笑的花粉虽能在柱头表面萌发,但花粉萌发和花粉管生长速度均慢于紫花含笑自交,且出现花粉管膨大、花粉管停止生长、柱头和花柱内胼胝质堆积等现象。紫花含笑×含笑、紫花含笑×深山含笑的坐果率和种子萌发率均达80%以上,表明杂交亲和性较好;而紫花含笑×阔瓣含笑的坐果率仅有11.1%,且杂种胚败育,受精前障碍可能是影响其杂交的主要原因之一。
To reveal the cross-compatibility of Michelia crassipes and that of other three species（M. figo,M. maudiae and M. platypetala）,M. crassipes was used as female parent and M. figo,M. maudiae and M. platypetala as male parent respectively for artificial hybridization. The pollen viability of the species was determined via culture in vitro,and the pollen tube germination after pollination was observed by fluorescence microscope. The fruit setting rate and seed quality of interspecific hybridization were investigated. The results indicated that the pollen viabilities of M. crassipes,M. figo and M. maudiae were above 65%,whereas the pollen viability of M. platypetala was only 29.07%. By fluorescence microscope,pollen germination rate and the pollen tube growth rate of M. crassipes × M. figo and M. crassipes × M. maudiae were higher than those of M. crassipes selfing. Pollen tubes could normally elongate to complete fertilization. The pollen grains of M. crassipes × M. platypetala germinated on the stigma,but the pollen germination and the growth rate of pollen tube were slower than that of the self-pollination,and inflated pollen tube,growth stop,and deposition of callose on stigma and in stylet were observed. The fruit setting rate and seed germination rate in the cross of M. crassipes × M. figo and M. crassipes × M. maudiae were above 80%,indicating that cross-compatibilities of M. crassipes with M. figo and M. maudiae were better. However,the fruit setting rate of M. crassipes × M. platypetala was only 11.1%,and hybrid embryo was abortive. The pre-fertilization barriers caused by the pollen tubes stopping growth could be one of the main barriers of interspecific hybridization between M. crassipes and M. platypetala.
作者
柴弋霞
胡希军
张冬林
刘晓玲
刘彩贤
金晓玲
CHAI Yixia;HU Xijun;ZHANG Donglin;LIU Xiaoling;LIU Caixian;JIN Xiaoling(College of LandscapeArchitecture,Central South University of Forestry and Technology,Changsha 410004,China;University of Georgia,Athens,GA 30602,USA)
出处
《园艺学报》
CAS
CSCD
北大核心
2018年第10期1970-1978,共9页
Acta Horticulturae Sinica
基金
国家林业公益性行业科研专项(201404710)
湖南省研究生科研创新项目(CX2017B403)
中南林业科技大学研究生科技创新基金资助项目(CX2017B11)
国家林业局“十三五”重点学科项目(林人发[2016]21号)
湖南省教育厅“十二五”重点学科项目(2011-76)
关键词
含笑属
紫花含笑
种间杂交
亲和性
坐果率
花粉管
Michelia crassipes
interspecific hybridization
cross-compatibility
fruiting rate
pollen tube
