摘要
为了解腐植酸(HA)对重度干旱胁迫燕麦叶片糖组分和内源激素的影响,以白燕2号为试验材料,利用盆栽设置不同水分和喷施腐植酸4个处理(正常供水,正常供水喷施腐植酸,重度干旱胁迫和重度干旱胁迫喷施腐植酸),利用高效气相色谱和质谱联用技术测定燕麦叶片可溶性糖和内源激素含量。结果表明:1)在燕麦叶片中检测到单糖和衍生物11种,低聚糖5种。通过主成分分析发现,不同处理之间差异主要体现在木糖、核糖、松三糖、葡萄糖、蔗糖和岩藻糖的含量。重度干旱胁迫喷施HA主要影响的是核糖和蔗糖,与CK相比,重度干旱胁迫核糖含量增加5.78%,蔗糖含量增加83.08%,差异显著(P<0.05,下同),重度干旱胁迫下喷施HA后,核糖和蔗糖含量均显著下降。2)与对照相比,重度干旱胁迫下,内源酸性激素脱落酸(ABA)、茉莉酸(JA)和水杨酸(SA)含量显著升高,生长素(IAA)含量显著降低,重度干旱胁迫下喷施HA后ABA、JA和SA含量显著降低。3)蔗糖含量与ABA、IAA含量显著相关,JA含量与细胞结构物质木糖、岩藻糖和核糖等戊糖含量均显著正相关,SA、ABA含量与糖信号物质果糖、葡萄糖含量显著负相关。可见,HA可以通过调控糖组分和内源激素缓解重度干旱胁迫伤害。
Oat variety Baiyan 2 was used as study object to investigate the effects of humic acid（HA）on the sugar components and endogenous hormones of oats under severe drought stress by HPGC-MS.Using potted plants,four treatments with different amounts of water and spraying humic acid（Normal water condition;Normal water condition spraying humic acid;Severe drought stress;Severe drought stress spraying humic acid）were set up.The results showed that：1）Ribose and sucrose were the main sugars of humic acid to relieve severe drought stress.Compared with CK,the ribose content of SS increased by 5.78%,sucrose increased by 83.08%,and the difference was significant（P〈0.05 the same below）.After spraying humic acid severe drought stress,sucrose and ribose decreased significantly.2）Compared with the control,the contents of ABA,JA and SA were significantly increased and the content of IAA significantly reduced under severe drought stress.Under severe drought stress after spraying humic acid,the contents of ABA,JA and SA were significantly decreased.3）The content of JA was positively correlated with xylose such as xylose,fucose and ribose.The correlation between SA and sugar was similar to that of ABA andsugar,and the content of SA was negatively correlated with fructose and glucose content.In conclusion,the humic acid could relieve severe drought stress by regulating soluble sugar components and endogenous hormones.
作者
张志芬
付晓峰
赵宝平
刘俊青
刘景辉
ZHANG Zhifen;FU Xiaofeng;ZHAO Baoping;LIU Junqing;LIU Jinghui(Cooperative Innovation Center of Grain Industry,Inner Mongolia Agricultural University Hohhot 010018 China;Inner Mongolia Grain Engineering Technology Research Center Hohhot 010018 China;Inner Mongolia Academy of Agriculture and Animal Husbandry,Hohhot 010031 China)
出处
《中国农业大学学报》
CAS
CSCD
北大核心
2018年第9期11-20,共10页
Journal of China Agricultural University
基金
国家燕麦荞麦产业技术体系(CARS-08-B-5
CARS-08-A-5)
国家自然科学基金项目(31560373)
内蒙古自然科学基金项目(2015MS0343)
