摘要
为探明中华小长臂虾对盐度的耐受性以及在不同盐度条件下的呼吸代谢,本实验在获得中华小长臂虾24～96 h的半致死盐度基础上,采用静水密闭式方法,从淡水直接突变到不同盐度(0、5、10、15、20、25、30)和放入不同盐度适应3天后(分别记为0 d组和3 d组),检验了盐度对中华小长臂虾耗氧率、排氨率和窒息点的影响。结果表明:中华小长臂虾对盐度的耐受能力较强,在24、48、72和96 h的半致死盐度分别为37.07、35.86、35.04和34.31,安全盐度为10.07;盐度对中华小长臂虾的耗氧率、排氨率、窒息点和O/N均具有显著影响(P<0.05);在盐度为0～30范围内,不论0 d组还是3 d组,耗氧率均随着盐度的增加出现先下降再增加再下降的变化趋势,0 d组的耗氧率在盐度为5时最低,盐度为10时最高,3 d组则在盐度10、25和30时最低,盐度为0时最高;排氨率在0 d组以盐度为30时最低,在盐度为10和15时最高,3 d组的排氨率则在盐度25和30时最低,盐度为10时最高;盐度对0 d组和3 d组的窒息点均没有显著影响; 0 d组的O/N最低值出现在盐度为5时,而3 d组则在盐度为10时,不同处理组的O/N最高值均是出现在盐度为30时;从相同盐度不同的处理方式来看,在盐度为10、15和25时,3 d组的耗氧率要显著低于0 d组(P<0.05);在盐度为25时,3 d组的中华小长臂虾排氨率显著低于0 d组,在盐度为30时,3 d组的中华小长臂虾O/N显著低于0 d组,其他处理组之间并没有显著性差异;中华小长臂虾对盐度具有较高的耐受性,能够在一定盐度的水体进行养殖,但不同的盐度和处理方式均会改变其呼吸代谢和能量利用的方式。
To investigate the salinity tolerance and respiratory metabolism of Palaemonetes sinen- s/s, we examined the effects of salinity （0, 5, 10, 15, 20, 25, 30） on survival, oxygen consumption rate （ OCR ） , ammonia excretion rate （ AER）, and asphyxiation point （AP） of P. sinensis which were exposed for 0 or 3 days in laboratory. The results showed that P. sinensis had strong salinity tolerance. The semi-lethal salinities were 37.07, 35.86, 35.04 and 34.31 at 24, 48, 72 and 96 h respectively, with a safe salinity concentration of 10.07. Salinity had significant effects on OCR, AER, AP and O/N ratio for P. sinensis （P〈 0. 05 ）. For the 0-day-exposure group, the OCR was lowest at salinity of 5 and highest at salinity of 10 when the salinity ranged from 0 to 30. For the 3-day-exposure group, the lowest OCR was at the salinity of 10, 25 and 30 while the highest OCR was salinity of O. For the O-day-exposure group, the lowest AER was at salinity of 30, and the highest was at salinity of 10 and 15. For the 3-day-exposure group, thelowest AER was at salinity of 25 and 30, and the highest was at salinity of 10. There was no significant difference of AP between 0-day-exposure and 3-day-exposure groups. The lowest O/N ratio was at salinity of 5 for the 0-day-exposure group and was at salinity of 10 for the 3-day-expo- sure group, while the O/N ratio was highest at salinity of 30 for both groups. The OCR in the 3- day-exposure group was lower than that in the 0-day-exposure group at salinity of 10, 15 and 25. The AER in the 3-day-exposure group was lower than that in the 0-day-exposure group at salinity of 25. The O/N ratio in the 3-day-exposure group was significantly lower than that of the 0-day- exposure group at salinity 30. Overall, P. sinensis had a high tolerance to salinity and could be cultured in water with a certain salinity. However, different salinities and treatment methods may change its respiratory metabolism and energy utilization.
作者
包杰
姜宏波
付佩佩
蒋秋月
于业辉
李晓东
BAO Jie;JIANG Hong-bo;Fu Pei-pei;JIANG Qiu-yue;YU Ye-hui;LI Xiao-dong(College of Animal Science and Veterinary Medicine,Shenyang Agricultural University,Shenyang 110866,China;Panjin Guanghe Crab Ltd.,Co.,Panjin 124220,Liaoning,China)
出处
《生态学杂志》
CAS
CSCD
北大核心
2018年第11期3351-3356,共6页
Chinese Journal of Ecology
基金
现代农业产业技术体系建设专项资金(CARS-48)
辽宁省博士启动基金项目(201601103)
辽宁省自然科学基金项目(20160637)资助
关键词
中华小长臂虾
盐度
耐受性
耗氧率
排氨率
窒息点
O/N比
Palaemonetes sinensis
salinity
tolerance
oxygen consumption rate
ammoniaexcretion rate
asphyxiation point
O/N ratio.
