摘要
为研究已建立的《规模化猪场猪瘟（CSF）和猪伪狂犬病（PR）净化方案》在猪场中的应用效果,本试验分4次采集了实施该方案猪场中的1 776份扁桃体和血液样品,并采用已建立的猪瘟病毒（CSFV）RT-PCR检测技术、伪狂犬病病毒（PRV）gE/gB-ELISA检测方法（GB/T18641-2002）、PRV野毒与疫苗毒多重PCR鉴别方法与生物安全措施,对贵州省某规模化种猪场CSF和PR开展了净化研究。结果显示,所检测PRV-gB抗体阳性率从83.98%上升到98.17%;PRV-gE抗体阳性率从5.72%下降到0.23%;PRV抗原阳性率从2.75%下降到0.00%;CSFV抗体阳性率从85.81%上升到98.63%;CSFV抗原阳性率从2.06%下降到0.00%,表明该净化技术应用效果确切。该研究为猪场CSF和PR的净化思路提供了一定的参考依据。
To investigate the application effect of ＂large-scale pig farm classical swine fever（CSF） and porcine pseudorabies （PRV）eradication program＂ in pig farms, 1 776 tonsils and blood sam- ples were collected from large-scale pig farm in four stages, and the RT-PCR technique for classical swine fever virus （ CSFV）, the pseudorabies virus （PRV） gE/gB-EI.ISA （ GB/T 18641-2002 ）, the multiplex PCR method for differentiation of PRV wild-type virus from vaccine strains and biosecu rity measures were carried out for the eradication evaluation of on CSF and PR in a large-scale pig farm in Guizhou Province. The results showed that the positive rate of anti-PRV-gB antibody in- creased from 83. 98% to 98. 17%, the positive rate of anti-PRV-gE antibody decreased from 5.72% to 0.23% ;the positive rate of PRV antigens decreased from 2.75% to 0.00% ;the positive rate of anti-CSFV antibody increased from 85.81% to 98.63% ;CSFV antigen positive rate was de- creased from 2.06% to 0.00% ,indicating that the effect of the eradication technology was exact. The study provided a reference for the purification of CSF and PR.
作者
胡玲玲
汤德元
曾智勇
王彬
黄涛
龙冬梅
石远菊
杨伟
叶丽
HU Ling-ling,TANG De-yuan ,ZENG Zhi-yong, WANG Bin, HUANG Tao, LONG Dong-mei, SHI Yuan-ju,YANG Wei,YE Li(College of Animal Science ,Ouizhou University ,Guiyang 550025, China)
出处
《中国兽医学报》
CAS
CSCD
北大核心
2018年第11期2058-2062,共5页
Chinese Journal of Veterinary Science
基金
贵州省2017年农业攻关资助项目(黔科合支撑[2017]2579号)
贵州省2014年农业攻关资助项目(黔科合NY字(2014)3055号)
