摘要
目的了解洛阳地区无偿献血人群中人类嗜T淋巴细胞病毒（human T-cell lymphotropic virus,HTLV）的感染情况。方法用双抗原夹心酶联免疫法（ELISA）筛查2016年3月1日至2016年10月22日期间采集的无偿献血者标本34 009份。对于筛查中发现的反应性标本（s/co≥1）再用核酸试验（PCR）和免疫印迹（WB）的检测方法进行确认。结果用ELISA方法共检出5份反应性标本,初筛反应率为0. 015%,再用PCR和WB方法检测5份初筛反应性标本,确认阳性结果 0份,确认阳性率为0%。结论从目前检测结果来看,暂未发现洛阳地区献血人群中有HTLV感染阳性,这可能与正常无偿献血者人群和绝大部分献血者属内陆地区人员等因素有关。
Objective To evaluate the current infection status of human T-cell lymphotrophic virus （ HTLV ） in the blood donors in Luoyang. Methods Screening of 34,009 voluntary blood donation samples were collected, recorded from March 1, 2016 to October 22, 2016 and double antibody sandwich enzyme-linked immunosorbent assay （ELISA） was performed on these samples to determine the infection status. The positive samples （S/CO ratio ≥1 ） found in screening procedure were further verified by polymerase chain reaction （PCR） and Western blot （WB）. Results A total of 5 Positive samples were detected by ELISA, the positive rate in screen procedure was 0. 015%. The 5 positive samples were then verified by PCR and WB methods. The number of positive results were confirmed to be 0, and the positive rate was 0%. Conclusion According to the current test resuhs, no positive HTLV infection was found in the blood donors in Luoyang. This may be caused by the fact that most of the regular donors are inland Chinese.
作者
陈善华
朱丽莉
李慧晓
吕冬冬
孙雪梅
CHEN Shanhua;ZHU Lili;LI Huixiao;LV Dongdong;SUN Xuemei(Luoyang Blood Center,Luoyang 471000,China)
出处
《中国输血杂志》
CAS
2018年第8期874-876,共3页
Chinese Journal of Blood Transfusion
关键词
无偿献血人群
人类嗜T淋巴细胞病毒
感染
voluntary unpaid blood donors
human T-cell lymphotropic virus
infection
