摘要
采用水解法用TiO2包覆上转换纳米粒子Na YF4：Yb,Tm@NaGdF4：Yb,然后修饰聚乙烯亚胺（PEI）和聚丙烯酸（PAA）并偶联叶酸（FA）,制备了叶酸受体靶向纳米光敏剂（NaYF4∶Yb,Tm@NaGdF4∶Yb@TiO2@PEIPAA-FA）。借助XRD和TEM表征NaYF4∶Yb,Tm@NaGdF4∶Yb@TiO2的物相和形貌,FTIR和Zeta电位证实有机成分的成功修饰,并测试了产物的上转换发光光谱。结果表明：在980 nm近红外光（NIR）下,纳米光敏剂存在下的1,3-二苯基异苯并呋喃溶液吸收光谱的降低证明了单线态氧的产生。此外,纳米光敏剂可以载带阿霉素（DOX）,最大载药率为50.8%,包封率为84.7%。载药后的纳米光敏剂中DOX释放对介质具有pH响应性,在NIR照射的酸性介质（pH=5.0）中12 h的累积缓释率为38.1%,远高于中性介质（pH=7.4）的10.4%。
Folate receptor-targeted nanophotosensitizer（NaYF4∶Yb,Tm@NaGdF4∶Yb@TiO2 @PEI-PAA-FA） was prepared by coating Ti O2 on the upconversion nanoparticles NaYF4∶Yb,Tm@NaGdF4∶Yb via a hydrolysis method, followed by modification with polyethyleneimine（PEI） and polyacrylic acid（PAA） and subsequent conjugation of folic acid（FA）. XRD and TEM were used to characterize the crystal structure and morphology of the inorganic components of the nanophotosensitizer. FTIR and Zeta potential were used to verify the binding of these organic components. Upconversion luminescence spectra of the samples were also measured. The decrease in the absorbance of 1,3-diphenylisobenzofuran solution in the presence of the nanophotosensitizer under 980 nm near-infrared（NIR） laser irradiation indicated the singlet oxygen was produced from the nanophotosensitizer. Besides, the photosensitizer could carry doxorubicin（DOX）, the maximum drug loading efficiency was 50.8% and the encapsulation efficiency was 84.7%. The DOX release from the nanophotosensitizer was sensitive to the p H value of the buffer. As the DOX-loaded nanophotosensitizer was suspended in an acidic buffer of pH=5.0 and exposed to NIR laser irradiation for 12 h, the cumulative release of DOX was 38.1%, much higher than that obtained from a neutral buffer（pH=7.4）, which was 10.4%.
作者
毛艳
梁天华
胡小锋
董孟杰
刘振锋
宫培军
MAO Yan;LIANG Tian-hua;HU Xiao-feng;DONG Meng-jie;LIU Zhen-feng;GONG Pei-jun(Key Laboratory of the Ministry of Education for Advanced Catalysis Materials,Zhejiang Normal University,Jinhua 321004,Zhejiang,China;PET Center,Department of Nuclear Medicine,the First Affiliated Hospital,College of Medicine,Zhejiang University,Hangzhou 310003,Zhejiang,China)
出处
《精细化工》
EI
CAS
CSCD
北大核心
2018年第11期1914-1920,共7页
Fine Chemicals
基金
浙江省公益技术应用研究计划项目(2016C31014)
浙江省自然科学基金(LY16H180002)
浙江师范大学“先进催化材料”教育部重点实验室和“固体表面反应化学”浙江省重点实验室开放课题基金(2017-15)
国家级大学生创新创业训练计划(201710345018)~~
关键词
上转换纳米粒子
二氧化钛
阿霉素
叶酸
药物缓释
光动力治疗
化疗
医药原料
upconversion nanoparticles
titanium dioxide
doxorubicin
folic acid
drug release
photodynamic therapy
chemotherapy
drug materials
