摘要
RAFT试剂介入制备白藜芦醇分子印迹聚合物并用于实际样品中白藜芦醇的分离富集。以二苄基三硫代碳酸酯(DBTTC)为RAFT试剂,以白藜芦醇为印迹化合物,以丙烯酰胺作为功能单体,利用可逆加成-断裂链转移自由基聚合法(RAFT)制备了白藜芦醇分子印迹聚合物。利用扫描电镜、红外光谱和色谱法考察了单体与交联剂比例、引发剂用量、溶剂用量、反应时间和反应温度对印迹聚合物形态结构、印迹聚合物的识别能力及分离效率的影响。结果显示,利用RAFT聚合法制备的白藜芦醇分子印迹聚合物对模板分子的结合量Q达到1 283μg/g,并具有特异性识别作用。通过活性自由基聚合法合成的分子印迹聚合物具有更好的形态结构,对目标分子具有较高的吸附效率。
The resveratrol imprinted polymer prepared by RAFT reagent was used for separation and enrichment of real sample. Resveratrol imprinted polymer was synthesized by a reversible addition-fragmentation chain transfer （RAFT） polymerization method, combining molecular imprinting with living radical polymerization techniques, using the dibenzyltrithiocarbonate （DBTTC） as reversible RAFT reagent, resveratrol as the imprinted compound, and acrylamide （AM） as functional monomers, ethyleneglycoldim ethacrylate （EDMA） as cross-linker and 2,2-azob isisobuty ronitrile （AIBN） as initiator. The effects of the ratio of monomer to cross-linker, initiator dosage, solvent dosage, reaction time and reaction temperature on the morphology, recognition ability and separation efficiency of imprinted polymers were investigated by scanning electron microscopy （SEM）,infrared spectroscopy （IR） and high performance liquid chromatography （HPLC）.Results indicated that the polymer was selective for resveratrol with a combined amount （ Q ） of 1 283μg/g. Living radical polymerization method was applied to synthesize the resveratrol imprinted polymer with a better morphology and a higher adsorption efficiency for the target.
作者
楚刚辉
吴坤
肖文清
CHU Ganghui;WU Kun;XIAO Wenqing(Xinjiang Laboratory of Native Medicinal and Edible Plant Resources Chemistry,College of Chemistry and Environmental Science,Kashgar University,Kashgar 844006,China)
出处
《功能材料》
EI
CAS
CSCD
北大核心
2018年第11期11127-11131,共5页
Journal of Functional Materials
基金
广东省科技计划资助项目(2014A020209082)
国家级大学生创新创业资助项目(201510763007)
关键词
活性自由基聚合
分子印迹聚合物
白藜芦醇
葡萄皮
高效液相色谱法
living radical polymerization
molecular imprinted polymer
resveratrol
grape skins
high performance liquid chromatography
