摘要
目的探讨受体相互作用蛋白2(receptor interacting protein 2,RIP2)基因对结直肠癌(colorectal cancer,CRC)肿瘤细胞侵袭、转移的影响及其作用机制。方法利用免疫组化染色(IHC)法检测CRC组织及癌旁正常组织中RIP2、基质裂解蛋白和明胶酶B的表达。分析患者病理情况、RIP2、基质裂解蛋白和明胶酶B的关联性;利用RNAi技术抑制CRC细胞株HCT116中RIP2的表达,比较转染前后肿瘤细胞的迁移能力。结果 CRC组细胞中RIP2、基质裂解蛋白和明胶酶B的表达明显高于正常对照组(P <0. 05); RIP2的表达与CRC细胞的浸润深度和淋巴结的转移程度有明显相关性(P <0. 05); CRC肿瘤细胞中RIP2、基质裂解蛋白、明胶酶B三者表达均呈正相关(P <0. 05);选择性抑制RIP2的表达后,肿瘤细胞的迁移能力明显降低,且基质裂解蛋白和明胶酶B的表达均明显降低。结论 CRC细胞中的RIP2能够通过上调基质裂解蛋白和明胶酶B的表达,提高CRC细胞的侵袭、转移能力。
Objective To investigate the effect and mechanism of receptor interacting protein 2 gene（ RIP2） on invasion and migration of colorectal cancer（ CRC）. Methods IHC method was used to detect the expressions of matrilysin,Gelatinase B and RIP2. The relevance among pathologic condition,matrilysin,Gelatinase B and RIP2 were analyzed. RNAi technology was used to inhibit the expression of RIP2 in HCT116 cells,and the ability of HCT116 cells in invasion and migration was detected. Results Compared with the normal group,the expressions of RIP2,matrilysin and Gelatinase B in CRC group were significantly higher（ P〈0. 05）. The expression of RIP2 was significantly related with invasion depth and lymph node metastasis（ P〈0. 05）. There was positive relationship among RIP2,matrilysin and Gelatinase B（ P〈0. 05）. After inhibition of RIP2,the migration ability of HCT116 cells was weakened,and expressions of matrilysin,Gelatinase B were decreased significantly. Conclusion The RIP2 in CRC cells can improve the invasion and metastasis ability by up-regulated expressions of matrilysin and Gelatinase B.
作者
李艳
徐智媛
程宇
杨晋辉
LI Yan;XU Zhiyuan;CHENG Yu;YANG Jinhui(Department of Gastroenterology,the Second Affiliated Hospital of Yunnan Medical University,Kunming 650101,China)
出处
《胃肠病学和肝病学杂志》
CAS
2018年第11期1239-1243,共5页
Chinese Journal of Gastroenterology and Hepatology
关键词
结直肠癌
基质裂解蛋白
受体相互作用蛋白2
明胶酶B
免疫组织化学
侵袭
转移
Colorectal cancer
Matrix metalloproteinases
Receptor interacting protein 2
Gelatinases B
Immunohistochemistry
Invasion
Metastasis
