摘要
目的:研究三氟淫羊藿素(Icaritfluor,ICTF)对脑缺血再灌注损伤的保护作用,初步探讨ICTF对GluN3A mRNA水平和蛋白表达的影响。方法:采用大鼠大脑中动脉栓塞(MACO)动物模型,TTC染色测定脑梗死范围,q-PCR方法检测GluN3A mRNA的表达,Western blot方法检测GluN3A蛋白表达水平。结果:MCAO再灌注模型组大鼠出现明显的脑梗死,缺血半损伤区脑组织GluN3A mRNA及蛋白表达均降低,给予ICTF后,脑梗死体积缩小,GluN3A mRNA及蛋白表达均升高。结论:ICTF对脑缺血再灌注损伤具有保护作用,其保护作用可能与升高NMDA受体的GluN3A亚基表达有关。
Objective： To study the protective effect of Trifluoro-icaritin （ICTF） on cerebral ischemia/reperfusion injury（I/RI）, and investigate the mRNA and protein expression of GluN3A content N-methyl-D-aspartate receptor （NMDAR）. Methods： The MCAO animal model in Sprague-Dawley rat was adopted, and the cerebral infarction range was determined by TTC staining. The expression of GluN3A mRNA and protein were detected by q-PCR and Western blot assay respectively. Results： The ischemia/reperfussion（I/R） model group showed an obvious cerebral infarction and decreases expression of GluN3A mRNA and protein in the ischemic semi-injury area. The volume of cerebral infarction was reduced and the expression of GluN3A mRNA and protein were increased after ICTF treatment. Conclusion： ICTF has an inhibition effect on cerebral I/R injury, which may be relevant with the increasing of GluN3Acontent NMDAR.
作者
刘松
刘超明
李顺益
俞家凡
刘瑞珍
黄志华
黄诚
李良东
LIU Song;LIU Chao-ming;LI Shun-yi;YU Jia-fan;LIU Rui-zhen;HUANG Zhi-hua;HUANG Chen;LI Liang-dong(Gannan Medical University： 1.graduate students of Grade 2016;undergraduate students of Grade 2014;School of Basic Medcine;The First Affiliated Hospital,Ganzhou,Jiangxi 341000)
出处
《赣南医学院学报》
2018年第10期972-976,共5页
JOURNAL OF GANNAN MEDICAL UNIVERSITY
基金
江西省自然科学基金项目(20161BAB205228)
江西省教育厅科技项目(GJJ70870)
赣南医学院研究生创新项目(YC2017-X003)
