摘要
聚合酶链式反应-变性梯度凝胶电泳(PCR-DGGE)技术已广泛应用于微生物分子生态学领域,成为研究微生物多样性常用的分子检测工具之一。在总结归纳PCR-DGGE技术的原理和特点的基础上,综述了PCR-DGGE技术在城镇供水系统和污水处理系统中微生物多样性分析等方面的研究进展,并展望了该技术今后的发展方向。
Polymerase Chain Reaction denaturing Gradient Gel Electrophoresis （PCR DGGE） technology has been widely applied in the field of microbial molecular ecology and has become one of the most popular molecular detection tools for microbial diversity research. On the basis of summarizing the principles and characteristics of PCR DGGE technology,this paper reviews the research progress of PCR DGGE in the a nalysis of microbial diversity in urban water supply and sewage treatment systems and looks forward to the future development direction of the technology.
作者
贾淑宇
张克峰
逯南南
王明泉
赵清华
孙韶华
贾瑞宝
JIA Shuyu;ZHANG Kefeng;LU Nannan;WANG Mingquan;ZHAO Qinghua;SUN Shaohua;JIA Ruibao(School of Municipal 1 F.nvironmental Engineering,Shanclong J ianzhu University,J inan 250101,China;Shandong Province City Water Supply and Drainage Water Quality Monitoring Centre,Jinan 250013,China)
出处
《安全与环境工程》
CAS
北大核心
2018年第6期60-66,共7页
Safety and Environmental Engineering
基金
国家水体污染控制与治理科技重大专项项目(2015ZX07406005)
山东省泰山学者建设工程专项项目(ts201712084)
泉城"5150"引才倍增计划项目
