摘要
用0.1g·L^(-1)氯金酸溶液100mL与10g·L^(-1)柠檬酸三钠溶液2mL反应制成红色纳米金颗粒(AuNPs)溶液,AuNPs粒径约为12nm,AuNPs溶液在波长518nm处有特征吸收峰。当三聚氰胺与AuNPs同处于一溶液中时,三聚氰胺的诱导作用使AuNPs团聚,其溶液的颜色由原来的红色变成蓝色(即在波长680nm附近出现新的吸收带)。三聚氰胺的检测范围为0.1～28.0μmol·L^(-1),测定下限(3σ)为0.1μmol·L^(-1)。据此,提出了测定牛奶中三聚氰胺含量的分光光度法。用标准加入法进行回收试验,回收率在99.0%～108%之间。
A red-colored solution of gold nanoparticles （AuNPs） with its particle diameter of about 12 nm was prepared by the reaction of 0.1 g · L^-1 H[AuC14] solution （100 mL） and 10 g · L^ -1 trisodium citrate solution （2 mL）. The AuNPs solution shows its characteristic absorption peak at the wavelength of 518 nm. When melamine was added to the AuNPs solution, agglomeration of AuNPs happened due to induction by melamine and the original red color of the AuNPs solution was turned to blue color, showing a new absorption band near 680 nm. Detection range of melamine was 0.1-28.0 μmol · L^ -1 , with lower limit （3σ） of determination 0.1 μmol · L^-1. Based on these facts, a spectrophotometric method for determination of melamine in milk was proposed. Test for recovery was made by standard addition method; values of recovery were in the range of 99.0%- 108%.
作者
孔英戈
张少云
潘萍
刘映
KONG Yingge, ZHANG Shaoyun, PAN Ping, LIU Ying(Hengyang Food and Drug Inspection and Testing Center, Hengyang 421001, China)
出处
《理化检验（化学分册）》
CAS
CSCD
北大核心
2018年第11期1303-1307,共5页
Physical Testing and Chemical Analysis(Part B:Chemical Analysis)
基金
湖南省科技成果项目(943Y20120450)
衡阳市农林卫生科研项目(2016KYA049)
关键词
分光光度法
纳米金
三聚氰胺
牛奶
spectrophotometry
gold nanoparticle
melamine
milk
