摘要
为提高预处理秸秆糖化效率,提高秸秆的营养价值,试验首先对纤维素酶和木聚糖酶酶学特性进行分析,再通过单因素和响应面设计进行最佳糖化条件优化。结果显示,纤维素酶和木聚糖酶的最佳酶解pH值为4.3,最佳温度为50℃。预处理秸秆最佳糖化条件为酶解时间82.08 h、液固比10.1、纤维素酶量32.30 FPU/g秸秆、木聚糖酶量50 U/g秸秆(包含在纤维素酶中),总还原糖含量达到最高,预测结果为572.77 mg/g干物质,与试验值560.09 mg/g干物质基本一致。这表明优化方案和回归模型建立成功,实现了预处理秸秆高效糖化。
This experiment was conducted to improve the saecharifieation efficiency and nutritional val- ue of pretreated e.orn straw. In this paper, the c.ellulase and xylanase characteristics were analyzed, and the single factor and ,＇esponse surface analysis were used to optimize the enzymatic hydrolysis con- ditions. The rest, h showed the optimal pH and temperature of enzymatic hydrolysis for the pretreated corn straw were 4.3 and 50 ℃, respectively. The optimal conditions tot saccharification of corn straw were adding cellulase 32.30 FPU/g （contains xylanase 50 U/g） with liquid-to-solid ratio of 10 ： 1 for 82.08 h. The experimental maximal reducing sug- ars yield was 560.09 rag/g, compared with pre- dicting results 572.77 mg/g. These results indi- cated that the optimized scheme and the regres- sion model were successfully established, and the pretreated straw was efficiently saccharified.
作者
王平
刘梦洁
刘超齐
常娟
尹清强
党晓伟
高天增
Wang Ping;Liu Mengjie;Liu Chaoqi;Chang Juan;Yin Qingqiang;Dang Xiaowei;Gao Tianzeng
出处
《饲料工业》
北大核心
2018年第22期49-54,共6页
Feed Industry
基金
国家自然科学基金资助项目[31702148]
河南省重大科技专项资助项目[171100110500]
河南省科技攻关资助项目[182102110062]
关键词
预处理秸秆
糖化
响应面法
还原糖
纤维素酶
木聚糖酶
pretreated straw
saccharification
response surface method
reducing sugar
cellulase
xylanase
