摘要
为比较烤烟烟叶在机械除湿仓储(磷化氢熏蒸杀虫、硫酰氟熏蒸杀虫)和气调仓储3种处理模式下,烟叶感官品质、常规化学成分和烟叶表面细菌多样性的差异,对样品分别进行磷化氢熏蒸杀虫机械除湿仓储、硫酰氟熏蒸杀虫机械除湿仓储和气调仓储处理。仓储24个月后取样检测烟叶常规化学成分,评价样品感官品质,提取烟叶表面细菌总DNA,通过巢式PCR扩增及变性梯度凝胶电泳法分析3种处理条件下仓储后相同来源烟叶表面的细菌多样性指标。结果显示:(1)相同来源的烟叶样品机械除湿加磷化氢熏蒸仓储感官品质综合指标优于气调和机械除湿加硫酰氟熏蒸仓储,不同来源的烟叶样品化学指标变化呈现出不同的规律,气调仓储烟叶样品的含水率保持最好、质量损失最少。(2)对于云南师宗C3F烟叶在3种处理条件下仓储24个月后,机械除湿加磷化氢熏蒸仓储条件下烟叶表面的细菌种类最多、多样性指数最大;在机械除湿加硫酰氟熏蒸仓储条件下烟叶表面的细菌多样性指数最小;在气调仓储方式下烟叶样品表面一些种类的细菌数量存在较大差异,说明烟叶表面细菌的均匀性较差。
This work studied the effects of three storage methods on the quality, routine chemical components and surface bacterial diversity of flue-cured tobacco. The three methods were Method 1, mechanical dehumidification combined with phosphine fumigation;Method 2, mechanical dehumidification combined with sulfuryl fluoride fumigation; Method 3, controlled atmosphere storage. Samples of redried flue-cured tobacco were stored under the three storage methods for 24 months before routine chemical components and sensory quality of the samples were analyzed. In addition, surface bacterial diversity of the samples was investigated by nested PCR and denaturing gradient gel electrophoresis （DGGE）. The results showed that：1） The sensory quality of tobacco from the same tobacco-planting areas stored with Method 1 was better than that with Method 2 or Method 3. The chemical indexes of tobacco from different tobacco-planting areas were different under the storage conditions. The tobacco stored with Method 3 had higher moisture contents and lower weight losses. 2） Tobacco leaves （C3F） from Shizong in Yunnan stored with Method 1 for 24 months had the highest surface bacterial diversity and diversity index, while those stored with Method 2 had the lowest diversity index. The amounts of surface bacteria stored with Method 3 were significantly different, which indicated that the distribution of bacteria on the surface of tobacco leaves was not even.
作者
王永红
赵敏
潘广乐
李小斌
刘帅东
冯兵孝
王宏岩
付瑜
王健强
吴成春
WANG Yonghong;ZHAO Min;PAN Guangle;LI Xiaobin;LIU Shuaidong;FENG Bingxiao;WANG Hongyan;FU Yu WANG Jianqiang;WU Chengchun(Technology Center,China Tobacco Shaanxi Industrial Co.,Ltd.,Baoji 721013,Shaanxi,China;Hanzhong Cigarette Factory,China Tobacco Shaanxi Industrial Co.,Ltd.,Hanzhong 723102,Shaanxi,China;Baoji Cigarette Factory,China Tobacco Shaanxi Industrial Co.,Ltd.,Baoji 721013,Shaanxi,China)
出处
《烟草科技》
EI
CAS
CSCD
北大核心
2018年第11期36-42,共7页
Tobacco Science & Technology
基金
陕西中烟工业有限责任公司科技项目"片烟仓储养护技术对比研究"(BA000-BJ14027)
关键词
烟叶
仓储
醇化
化学成分
细菌多样性
巢式PCR
Tobacco leaf
Storage
Aging
Chemical component
Bacterial diversity
Nested PCR
